ArticleThe EMBO journal2021
Endospore Appendages: a novel pilus superfamily from the endospores of pathogenic Bacilli.
Article in The EMBO journal, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 34 citations in OpenAlex.
- Purification and Thioflavin T Staining of Bacillus spp. Endospores for Fluorescence-Based Assays.Current protocols · 2026Article
- Matrix plasticity and the molecular basis of extracellular filament assembly inScience advances · 2026Article
- Auto-crosslinking sporesilk fibers promote endospore and Cry toxin clustering.Nature communications · 2026Article
- Spatiotemporal expression of endospore appendages and cryo-EM insights into Ena1C-mediated S-ENA anchoring in Bacillus paranthracis.Scientific reports · 2026Article
- Endospore appendages enhance adhesion ofApplied and environmental microbiology · 2025Article
- Cryo-EM identifies F-ENA of Bacillus thuringiensis as a widespread family of endospore appendages across Firmicutes.Nature communications · 2025Article
- Cryo-EM analysis of the Bacillus thuringiensis extrasporal matrix identifies F-ENA as a widespread family of endospore appendages across Firmicutes.Research square · 2025Article
- Cryo-EM analysis of thebioRxiv : the preprint server for biology · 2025Article
- Molecular architecture of the assembly of Bacillus spore coat protein GerQ revealed by cryo-EM.Nature communications · 2024Article
- Helical ultrastructure of the L-ENA spore aggregation factor of a Bacillus paranthracis foodborne outbreak strain.Nature communications · 2024Article
- Endospore pili: Flexible, stiff, and sticky nanofibers.Biophysical journal · 2023Article
- Mechanism of assembly of type 4 filaments: everything you always wanted to know (but were afraid to ask).Microbiology (Reading, England) · 2023Review
- Enigmatic Pilus-Like Endospore Appendages ofInternational journal of molecular sciences · 2021Review
- Endospore Appendages: a novel pilus superfamily from the endospores of pathogenic Bacilli.The EMBO journal · 2021Article
- Structure Determination of Microtubules and Pili: Past, Present, and Future Directions.Frontiers in molecular biosciences · 2021Review
Corrections and comments
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Authors and funding
10 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Bacillus cereus sensu lato is a group of Gram-positive endospore-forming bacteria with high ecological diversity. Their endospores are decorated with micrometer-long appendages of unknown identity and function. Here, we isolate endospore appendages (Enas) from the food poisoning outbreak strain B. cereus NVH 0075-95 and find proteinaceous fibers of two main morphologies: S- and L-Ena. By using cryoEM and 3D helical reconstruction of S-Enas, we show these to represent a novel class of Gram-positive pili. S-Enas consist of single domain subunits with jellyroll topology that are laterally stacked by β-sheet augmentation. S-Enas are longitudinally stabilized by disulfide bonding through N-terminal connector peptides that bridge the helical turns. Together, this results in flexible pili that are highly resistant to heat, drought, and chemical damage. Phylogenomic analysis reveals a ubiquitous presence of the ena-gene cluster in the B. cereus group, which include species of clinical, environmental, and food importance. We propose Enas to represent a new class of pili specifically adapted to the harsh conditions encountered by bacterial spores.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.