ArticleJournal of assisted reproduction and genetics2021
Effect of oxygen and glucose availability during in vitro maturation of bovine oocytes on development and gene expression.
Article in Journal of assisted reproduction and genetics, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
12 citing papers in PubMed, 17 citations in OpenAlex.
- A transcriptomic map of ligand-receptor interactions within bovine antral follicles predicts intrafollicular factors for improving oocyte quality.Journal of animal science and biotechnology · 2026Article
- Advances in bovine oocyte in vitro maturation: mechanisms and strategies.Veterinary research communications · 2026Review
- Use of low versus high oxygen tension during pre-maturation only in capacitation in vitro maturation (CAPA-IVM): impact on oocyte maturation and metabolism.Journal of assisted reproduction and genetics · 2026Article
- Impacts of Oxygen Tension on Developmental Competence of Preimplantation Embryos.Biomolecules · 2026Review
- Regulation of ovarian function in domestic animals: translational insights into human ovarian dynamics and dysfunction.Frontiers in endocrinology · 2026Review
- Effect of Oxygen Tension Modification During Oocyte Maturation on Porcine Oocyte Quality.Veterinary sciences · 2025Article
- Impact of low versus high oxygen tension on human oocyte maturation during biphasic capacitation IVM (CAPA-IVM).Journal of assisted reproduction and genetics · 2025Article
- Oxygen level alters energy metabolism in bovine preimplantation embryos.Scientific reports · 2025Article
- An Overview of Reactive Oxygen Species Damage Occurring during In Vitro Bovine Oocyte and Embryo Development and the Efficacy of Antioxidant Use to Limit These Adverse Effects.Animals : an open access journal from MDPI · 2024Review
- Alternative Culture Systems for Bovine Oocyte In Vitro Maturation: Liquid Marbles and Differentially Shaped 96-Well Plates.Animals : an open access journal from MDPI · 2023Article
- The Role of Mitochondria in Oocyte Maturation.Cells · 2021Review
- Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
purposeOxygen tension during the in vitro maturation (IVM) of oocytes is important for oocyte developmental competence. A conflict exists in the literature as to whether low oxygen during IVM is detrimental or beneficial to the oocyte. Many research and clinical labs use higher than physiological oxygen tension perhaps believing that low-oxygen tension is detrimental to oocyte development. Other studies show that glucose is important if low-oxygen tension is used during maturation. In this study, we look at the link between low oxygen and glucose availability during IVM to resolve misconceptions around low-oxygen tension during IVM.
methodsBovine cumulus oocyte complexes (COCs) were matured at 20% vs 7% oxygen in media containing differing glucose concentrations or varying availability. Cleavage and blastocyst rates were recorded. RT-PCR determined expression levels of metabolic, oxygen, and stress-responsive genes following IVM.
resultsEmbryo development in 7% oxygen groups with 2.3mM glucose/low glucose availability was lower than 20% oxygen groups. Under 7% oxygen with 5.6mM glucose or higher glucose availability, rates were restored to those seen in 20% oxygen. Expressions of BNIP3, ENO1, GAPDH, and SLC2A1, were upregulated in 7% oxygen/low glucose, compared to 20% oxygen groups. BNIP3 expression was higher in 7% oxygen group with low glucose availability compared to the 20% groups.
conclusionOocyte developmental competence is negatively impacted following IVM in low oxygen when glucose availability is limited. Glucose concentration and physical culture conditions need to be considered when comparing the effects of different oxygen concentrations during IVM.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.