ArticleScientific reports2021
Refinement of 16S rRNA gene analysis for low biomass biospecimens.
Article in Scientific reports, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed.
- Systematic quantification and removal of host DNA contamination in 16S rRNA gene sequencing.Quantitative biology (Beijing, China) · 2026Article
- A Scalable and Cost-Effective In-Line Barcoding Strategy for Standardized 16S rRNA Gene Amplicon Sequencing: Performance Evaluation and Bias Assessment.Molecular ecology resources · 2026Article
- Methodological bias shapes the interpretation of the urinary microbiome in low-biomass systems.Frontiers in microbiology · 2026Review
- Do we need a standardized 16S rRNA gene amplicon sequencing analysis protocol for poultry microbiota research?Poultry science · 2025Review
- Comparison of RNA- and DNA-based 16S amplicon sequencing to find the optimal approach for the analysis of the uterine microbiome.Scientific reports · 2025Article
- Age matters: exploring differential effects of antimicrobial treatment on gut microbiota of adult and juvenile brown trout (Salmo trutta).Animal microbiome · 2025Article
- Differences in cell-associated and cell-free microbial DNA in blood.bioRxiv : the preprint server for biology · 2025Article
- Illumina Sequencing in Conjunction with Propidium Monoazide to Identify Live Bacteria After Antiseptic Treatment in a Complex Oral Biofilm: A Study Using an Ex Vivo Supragingival Biofilm Model.Antibiotics (Basel, Switzerland) · 2024Article
- Long journey of 16S rRNA-amplicon sequencing toward cell-based functional bacterial microbiota characterization.iMetaOmics · 2024Review
- Regional Differences in Microbial Infiltration of Brain Tissue from Alzheimer's Disease Patients and Control Individuals.Brain sciences · 2024Article
- The seeds of Plantago lanceolata comprise a stable core microbiome along a plant richness gradient.Environmental microbiome · 2024Article
- The role of the microbiome in allergic dermatitis-related otitis externa: a multi-species comparative review.Frontiers in veterinary science · 2024Review
- Evaluation of fecal sample collection methods for feline gut microbiome profiling: fecal loop vs. litter box.Frontiers in microbiology · 2024Article
- Article
- Is there a placental microbiota? A critical review and re-analysis of published placental microbiota datasets.BMC microbiology · 2023Review
- Best practice for wildlife gut microbiome research: A comprehensive review of methodology for 16S rRNA gene investigations.Frontiers in microbiology · 2023Review
- Topical Glaucoma Therapy Is Associated With Alterations of the Ocular Surface Microbiome.Investigative ophthalmology & visual science · 2022Article
- The microbiome of common bedding materials before and after use on commercial dairy farms.Animal microbiome · 2022Article
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
High-throughput phylogenetic 16S rRNA gene analysis has permitted to thoroughly delve into microbial community complexity and to understand host-microbiota interactions in health and disease. The analysis comprises sample collection and storage, genomic DNA extraction, 16S rRNA gene amplification, high-throughput amplicon sequencing and bioinformatic analysis. Low biomass microbiota samples (e.g. biopsies, tissue swabs and lavages) are receiving increasing attention, but optimal standardization for analysis of low biomass samples has yet to be developed. Here we tested the lower bacterial concentration required to perform 16S rRNA gene analysis using three different DNA extraction protocols, three different mechanical lysing series and two different PCR protocols. A mock microbiota community standard and low biomass samples (10
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