ArticleNature communications2021
Fast widefield scan provides tunable and uniform illumination optimizing super-resolution microscopy on large fields.
Article in Nature communications, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 67 citations in OpenAlex.
- Brightness demixing for simultaneous multi-target imaging in 3D single-molecule localization microscopy.Nature methods · 2026Article
- Accelerated Left-Handed DNA-PAINT Using Fluorogenic Probes.Nano letters · 2026Article
- Impact of uniform illumination in widefield microscopy and mesoscopy.Scientific reports · 2025Article
- Enabling real-time reconstruction for large field-of-view single-molecule localization microscopy using discrete field-dependent point-spread function.Biomedical optics express · 2025Article
- Unveiling the cell biology of hippocampal neurons with dendritic axon origin.The Journal of cell biology · 2025Article
- Calibration-free estimation of field dependent aberrations for single molecule localization microscopy across large fields of view.bioRxiv : the preprint server for biology · 2024Article
- Article
- Photonic lantern TIRF microscopy for highly efficient, uniform, artifact-free imaging.Optics express · 2024Article
- Frontiers in artificial intelligence-directed light-sheet microscopy for uncovering biological phenomena and multi-organ imaging.View (Beijing, China) · 2024Article
- Build and operation of a custom 3D, multicolor, single-molecule localization microscope.Nature protocols · 2024Review
- Correlative single-molecule and structured illumination microscopy of fast dynamics at the plasma membrane.Nature communications · 2024Article
- Multimodal illumination platform for 3D single-molecule super-resolution imaging throughout mammalian cells.Biomedical optics express · 2024Article
- Multimodal illumination platform for 3D single-molecule super-resolution imaging throughout mammalian cells.bioRxiv : the preprint server for biology · 2024Article
- Multidimensional Widefield Infrared-Encoded Spontaneous Emission Microscopy: Distinguishing Chromophores by Ultrashort Infrared Pulses.Journal of the American Chemical Society · 2024Article
- Multiplexed and Millimeter-Scale Fluorescence Nanoscopy of Cells and Tissue Sections via Prism-Illumination and Microfluidics-Enhanced DNA-PAINT.Chemical & biomedical imaging · 2023Article
- Assessing crosstalk in simultaneous multicolor single-molecule localization microscopy.Cell reports methods · 2023Article
- Fibroblasts generate topographical cues that steer cancer cell migration.Science advances · 2023Article
- Article
- Error-Correction Method for High-Throughput Sizing of Nanoscale Vesicles with Single-Molecule Localization Microscopy.The journal of physical chemistry. B · 2023Article
- An open-source microscopy framework for simultaneous control of image acquisition, reconstruction, and analysis.HardwareX · 2023Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Non-uniform illumination limits quantitative analyses of fluorescence imaging techniques. In particular, single molecule localization microscopy (SMLM) relies on high irradiances, but conventional Gaussian-shaped laser illumination restricts the usable field of view to around 40 µm × 40 µm. We present Adaptable Scanning for Tunable Excitation Regions (ASTER), a versatile illumination technique that generates uniform and adaptable illumination. ASTER is also highly compatible with optical sectioning techniques such as total internal reflection fluorescence (TIRF). For SMLM, ASTER delivers homogeneous blinking kinetics at reasonable laser power over fields-of-view up to 200 µm × 200 µm. We demonstrate that ASTER improves clustering analysis and nanoscopic size measurements by imaging nanorulers, microtubules and clathrin-coated pits in COS-7 cells, and β2-spectrin in neurons. ASTER's sharp and quantitative illumination paves the way for high-throughput quantification of biological structures and processes in classical and super-resolution fluorescence microscopies.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.