Evidence map›Paper›PMID 34029631›Full record

ArticleJournal of controlled release : official journal of the Controlled Release Society2021

Minicircles for a two-step blood biomarker and PET imaging early cancer detection strategy.

Elise R Robinson, Gayatri Gowrishankar, Aloma L D'Souza, Azadeh Kheirolomoom, Tom Haywood, Sharon S Hori, Hui-Yen Chuang, Yitian Zeng, Spencer K Tumbale, Amin Aalipour and 7 more

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of controlled release : official journal of the Controlled Release Society, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
0.5field-weighted citation impact, top 38% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 7 citations in OpenAlex.

  1. Review
  2. Article
  3. Targeting and monitoring ovarian cancer invasion with an RNAi and peptide delivery system.Proceedings of the National Academy of Sciences of the United States of America · 2024
    Article
  4. Article
  5. Review
  6. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

17 authors at 3 institutions in 2 countries.

Elise R RobinsonDepartment of Bioengineering, Stanford University School of Medicine, Stanford, CA 94305, USA; Department of Radiology, Molecular Imaging Program at Stanford (MIPS), Stanford University School of Medicine, Stanford, CA 94305, USA.
Gayatri GowrishankarDepartment of Radiology, Molecular Imaging Program at Stanford (MIPS), Stanford University School of Medicine, Stanford, CA 94305, USA.
Aloma L D'SouzaDepartment of Radiology, Molecular Imaging Program at Stanford (MIPS), Stanford University School of Medicine, Stanford, CA 94305, USA.
Azadeh KheirolomoomDepartment of Radiology, Molecular Imaging Program at Stanford (MIPS), Stanford University School of Medicine, Stanford, CA 94305, USA.
Tom HaywoodDepartment of Radiology, Molecular Imaging Program at Stanford (MIPS), Stanford University School of Medicine, Stanford, CA 94305, USA.
Sharon S HoriDepartment of Radiology, Molecular Imaging Program at Stanford (MIPS), Stanford University School of Medicine, Stanford, CA 94305, USA; Canary Center at Stanford for Cancer Early Detection, Stanford University School of Medicine, Palo Alto, CA 94304, USA.
Hui-Yen ChuangDepartment of Biomedical Imaging and Radiological Sciences, National Yang Ming University, Taipei, Taiwan.
Yitian ZengDepartment of Materials Science and Engineering, Stanford University, Stanford, CA 94305, USA.
Spencer K TumbaleDepartment of Radiology, Molecular Imaging Program at Stanford (MIPS), Stanford University School of Medicine, Stanford, CA 94305, USA.
Amin AalipourDepartment of Bioengineering, Stanford University School of Medicine, Stanford, CA 94305, USA; Department of Radiology, Molecular Imaging Program at Stanford (MIPS), Stanford University School of Medicine, Stanford, CA 94305, USA.
Corinne BeinatDepartment of Radiology, Molecular Imaging Program at Stanford (MIPS), Stanford University School of Medicine, Stanford, CA 94305, USA.
Israt S AlamDepartment of Radiology, Molecular Imaging Program at Stanford (MIPS), Stanford University School of Medicine, Stanford, CA 94305, USA.
Ataya SathirachindaDepartment of Radiology, Molecular Imaging Program at Stanford (MIPS), Stanford University School of Medicine, Stanford, CA 94305, USA.
Masamitsu KanadaInstitute for Quantitative Health Science and Engineering (IQ), Michigan State University, East Lansing, MI 48824., USA.
Ramasamy PaulmuruganDepartment of Radiology, Molecular Imaging Program at Stanford (MIPS), Stanford University School of Medicine, Stanford, CA 94305, USA; Canary Center at Stanford for Cancer Early Detection, Stanford University School of Medicine, Palo Alto, CA 94304, USA.
Katherine W FerraraDepartment of Radiology, Molecular Imaging Program at Stanford (MIPS), Stanford University School of Medicine, Stanford, CA 94305, USA; Canary Center at Stanford for Cancer Early Detection, Stanford University School of Medicine, Palo Alto, CA 94304, USA. Electronic address: kwferrar@stanford.edu.
Sanjiv S GambhirDepartment of Radiology, Molecular Imaging Program at Stanford (MIPS), Stanford University School of Medicine, Stanford, CA 94305, USA; Canary Center at Stanford for Cancer Early Detection, Stanford University School of Medicine, Palo Alto, CA 94304, USA.
Stanford University · USMichigan State University · USNational Yang Ming Chiao Tung University · TW

Funding

Specific and high-resolution ultrasound cancer imagingR01CA112356 · NCI · UNIVERSITY OF CALIFORNIA DAVIS · PI FERRARA, KATHERINE W, FOIRET, JOSQUIN · 2005 to 2022
$6.1M
Reporter Imaging of Protein-Protein InteractionsR01CA082214 · NCI · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI GAMBHIR, SANJIV S · 1999 to 2019
$5.9M
In vivo PET imaging of novel engineered AAVs informs capsid design in Alzheimer's DiseaseR01EB028646 · NIBIB · STANFORD UNIVERSITY · PI FERRARA, KATHERINE W · 2019 to 2022
$3.8M
Predoctoral Training in Biomedical Imaging at Stanford UniversityT32EB009653 · NIBIB · STANFORD UNIVERSITY · PI BUTTS-PAULY, KIM · 2010 to 2019
$2.1M
NCI NIH HHS R01 CA082214NCI NIH HHS R01 CA112356NIBIB NIH HHS R01 EB028646NIBIB NIH HHS T32 EB009653
6 · The paper itself

Abstract

Early cancer detection can dramatically increase treatment options and survival rates for patients, yet detection of early-stage tumors remains difficult. Here, we demonstrate a two-step strategy to detect and locate cancerous lesions by delivering tumor-activatable minicircle (MC) plasmids encoding a combination of blood-based and imaging reporter genes to tumor cells. We genetically engineered the MCs, under the control of the pan-tumor-specific Survivin promoter, to encode: 1) Gaussia Luciferase (GLuc), a secreted biomarker that can be easily assayed in blood samples; and 2) Herpes Simplex Virus Type 1 Thymidine Kinase mutant (HSV-1 sr39TK), a PET reporter gene that can be used for highly sensitive and quantitative imaging of the tumor location. We evaluated two methods of MC delivery, complexing the MCs with the chemical transfection reagent jetPEI or encapsulating the MCs in extracellular vesicles (EVs) derived from a human cervical cancer HeLa cell line. MCs delivered by EVs or jetPEI yielded significant expression of the reporter genes in cell culture versus MCs delivered without a transfection reagent. Secreted GLuc correlated with HSV-1 sr39TK expression with R

Indexed as

NeoplasmsThymidine KinaseAnimalsBiomarkersGenes, ReporterHeLa CellsHumansPositron-Emission TomographyTransfectionBiomarkersThymidine KinaseBiomarkersCancer detectionMinicirclesReporter genes

Identifiers

PMID34029631
PMCPMC8262353
OpenAlexW3165070133

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.