ArticleMethods in molecular biology (Clifton, N.J.)2021
Transformation of the Plastid Genome in Tobacco: The Model System for Chloroplast Genome Engineering.
Article in Methods in molecular biology (Clifton, N.J.), 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed, 11 citations in OpenAlex.
- Expanding the palette of cis-elements to tune the expression of chloroplast transgenes in tobacco.The Plant journal : for cell and molecular biology · 2026Article
- Rewinding evolution in planta: A Rubisco-null platform validates high-performance ancestral enzymes.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Sustainable production of myoglobin meat protein in plant chloroplasts.Frontiers in plant science · 2026Article
- The psbA open reading frame acts in cis to toggle HCF173 from an activator to a repressor for light-regulated psbA translation in plants.The Plant cell · 2025Article
- Regulation of nucleus-encoded trans-acting factors allows orthogonal fine-tuning of multiple transgenes in the chloroplast of Chlamydomonas reinhardtii.Plant biotechnology journal · 2025Article
- Chloroplast Cell-Free Systems from Different Plant Species as a Rapid Prototyping Platform.ACS synthetic biology · 2024Article
- Carotenoid Pathway Engineering in Tobacco Chloroplast Using a Synthetic Operon.Molecular biotechnology · 2023Article
- A CRR2-Dependent sRNA Sequence Supports Papillomavirus Vaccine Expression in Tobacco Chloroplasts.Metabolites · 2023Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The protocol we report here is based on biolistic delivery of transforming DNA to tobacco leaves, selection of transplastomic clones by spectinomycin or kanamycin resistance and regeneration of plants with uniformly transformed plastid genomes. Because the plastid genome of Nicotiana tabacum derives from Nicotiana sylvestris, and the two genomes are highly conserved, vectors developed for N. tabacum can be used in N. sylvestris. The tissue culture responses of N. tabacum cv. Petit Havana and N. sylvestris accession TW137 are similar. Plastid transformation in a subset of N. tabacum cultivars and in Nicotiana benthamiana requires adjustment of the tissue culture protocol. We describe updated vectors targeting insertions in the unique and repeated regions of the plastid genome, vectors suitable for regulated gene expression by the engineered PPR10 RNA binding protein as well as systems for marker gene excision.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.