Evidence map›Paper›PMID 34028738›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2021

Techniques to Assess Collagen Synthesis, Deposition, and Cross-Linking In Vitro.

Tamara Rosell-García, Fernando Rodriguez-Pascual

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors.

Tamara Rosell-GarcíaCentro de Biología Molecular "Severo Ochoa" Consejo Superior de Investigaciones Científicas (C.S.I.C.), Universidad Autónoma de Madrid, Madrid, Spain.
Fernando Rodriguez-PascualCentro de Biología Molecular "Severo Ochoa" Consejo Superior de Investigaciones Científicas (C.S.I.C.), Universidad Autónoma de Madrid, Madrid, Spain. frodriguez@cbm.csic.es.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Synthesis, deposition, and cross-linking of collagen are hallmarks of fibroblast to myofibroblast differentiation. Standard methods for determining collagen from tissue samples are not directly applicable to cell culture conditions, where the overall synthesis and deposition of collagen is clearly unfavorable, mainly due to quantity limitations and dilution of required extracellular remodeling factors. In this chapter, we describe the methods we have established to analyze collagen production and deposition into the extracellular matrix by cultured myo/fibroblasts, as well as to determine lysyl oxidase (LOX) activity in cell supernatants as an index of the capacity of the cell to cross-link collagen in vitro.

Indexed as

AnimalsCell DifferentiationCell LineCollagenCross-Linking ReagentsExtracellular MatrixFibroblastsHumansMiceMyofibroblastsNIH 3T3 CellsProtein-Lysine 6-OxidaseCollagenCross-Linking ReagentsProtein-Lysine 6-OxidaseCollagen cross-linkingCollagen depositionExtracellular matrixFibroblastLysyl oxidase

Identifiers

PMID34028738

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.