Evidence map›Paper›PMID 34028720›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2021

High-Throughput Imaging of Arrays of Fluorescently Tagged Yeast Mutant Strains.

Mojca Mattiazzi Usaj, Dara S Lo, Ben T Grys, Brenda J Andrews

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.5field-weighted citation impact, top 40% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 2 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 2 institutions in 1 country.

Mojca Mattiazzi UsajThe Donnelly Centre, University of Toronto, Toronto, ON, Canada.
Dara S LoThe Donnelly Centre, University of Toronto, Toronto, ON, Canada.
Ben T GrysThe Donnelly Centre, University of Toronto, Toronto, ON, Canada.
Brenda J AndrewsThe Donnelly Centre, University of Toronto, Toronto, ON, Canada. brenda.andrews@utoronto.ca.
University of Toronto · CAToronto Metropolitan University · CA

Funding

Mapping the reference genetic network of a eukaryotic cellR01HG005853 · NHGRI · UNIVERSITY OF TORONTO · PI ANDREWS, BRENDA JEAN, BOONE, CHARLES · 2010 to 2025
$7.6M
CIHR FDN-143264NHGRI NIH HHS R01 HG005853
6 · The paper itself

Abstract

We describe a protocol for live-cell high-throughput (HTP) screening of yeast mutant strains carrying fluorescent protein markers for subcellular compartments of choice using automated confocal microscopy. This procedure, which combines HTP genetics and microscopy, results in the acquisition of thousands of images that can be analyzed in a systematic and quantitative way to identify morphology defects in the tagged subcellular compartments. This HTP protocol is readily adapted for screening any combination of markers and can be expanded to different growth conditions or higher order mutant genetic backgrounds.

Indexed as

MutationFluorescent DyesHigh-Throughput Screening AssaysMicroscopy, ConfocalMicroscopy, FluorescenceSaccharomyces cerevisiaeSaccharomyces cerevisiae ProteinsFluorescent DyesSaccharomyces cerevisiae ProteinsConfocal fluorescence microscopyGenetic screeningHigh-content screeningHigh-throughput microscopyYeast

Identifiers

PMID34028720
PMCPMC9107603
OpenAlexW3164935617

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.