ArticleBioengineering & translational medicine2021
Transcriptomic response of brain tissue to focused ultrasound-mediated blood-brain barrier disruption depends strongly on anesthesia.
Article in Bioengineering & translational medicine, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 22 citations in OpenAlex.
- Genomic Deletion of P-Glycoprotein from Gliomas Via the Focused Ultrasound-Delivery of a Duplex CRISPR Cas9 Ribonucleoprotein System.Cell biomaterials · 2026Article
- Narrowing of glioma vascular caliber via chronic VEGFR2 blockade improves the uniformity of focused ultrasound-mediated small molecule drug delivery.Journal of controlled release : official journal of the Controlled Release Society · 2026Article
- Pulsed focused ultrasound alters the proteomic profile of the tumor microenvironment in a syngeneic mouse model of glioblastoma.Journal of neuro-oncology · 2024Article
- Acoustically targeted measurement of transgene expression in the brain.Science advances · 2024Article
- Comprehensive assessment of blood-brain barrier opening and sterile inflammatory response: unraveling the therapeutic window.Scientific reports · 2024Article
- Research progress on the regulation and mechanism of borneol on the blood-brain barrier in pathological states: a narrative review focused on ischemic stroke and cerebral glioma.Translational cancer research · 2023Review
- Barriers to immune cell infiltration in tumors.Journal for immunotherapy of cancer · 2023Review
- Transcriptional signature in microglia isolated from an Alzheimer's disease mouse model treated with scanning ultrasound.Bioengineering & translational medicine · 2023Article
- The new insight into the inflammatory response following focused ultrasound-mediated blood-brain barrier disruption.Fluids and barriers of the CNS · 2022Article
- Spatially targeted brain cancer immunotherapy with closed-loop controlled focused ultrasound and immune checkpoint blockade.Science advances · 2022Article
- Neuroinflammation associated with ultrasound-mediated permeabilization of the blood-brain barrier.Trends in neurosciences · 2022Review
- The roles of thermal and mechanical stress in focused ultrasound-mediated immunomodulation and immunotherapy for central nervous system tumors.Journal of neuro-oncology · 2022Review
- Profiling of the immune landscape in murine glioblastoma following blood brain/tumor barrier disruption with MR image-guided focused ultrasound.Journal of neuro-oncology · 2022Article
- Noninvasive Low-Intensity Focused Ultrasound Mediates Tissue Protection following Ischemic Stroke.BME frontiers · 2022Article
- Low-Intensity Focused Ultrasound Technique in Glioblastoma Multiforme Treatment.Frontiers in oncology · 2022Review
- Functional Connectivity of the Brain Across Rodents and Humans.Frontiers in neuroscience · 2022Review
- Cavitation-modulated inflammatory response following focused ultrasound blood-brain barrier opening.Journal of controlled release : official journal of the Controlled Release Society · 2021Article
- Transcriptomic response of brain tissue to focused ultrasound-mediated blood-brain barrier disruption depends strongly on anesthesia.Bioengineering & translational medicine · 2021Article
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8 authors at 1 institution in 1 country.
Funding
Abstract
Focused ultrasound (FUS) mediated blood-brain barrier disruption (BBBD) targets the delivery of systemically-administered therapeutics to the central nervous system. Preclinical investigations of BBBD have been performed on different anesthetic backgrounds; however, the influence of the choice of anesthetic on the molecular response to BBBD is unknown, despite its potential to critically affect interpretation of experimental therapeutic outcomes. Here, using bulk RNA sequencing, we comprehensively examined the transcriptomic response of both normal brain tissue and brain tissue exposed to FUS-induced BBBD in mice anesthetized with either isoflurane with medical air (Iso) or ketamine/dexmedetomidine (KD). In normal murine brain tissue, Iso alone elicited minimal differential gene expression (DGE) and repressed pathways associated with neuronal signaling. KD alone, however, led to massive DGE and enrichment of pathways associated with protein synthesis. In brain tissue exposed to BBBD (1 MHz, 0.5 Hz pulse repetition frequency, 0.4 MPa peak-negative pressure), we systematically evaluated the relative effects of anesthesia, microbubbles, and FUS on the transcriptome. Of particular interest, we observed that gene sets associated with sterile inflammatory responses and cell-cell junctional activity were induced by BBBD, regardless of the choice of anesthesia. Meanwhile, gene sets associated with metabolism, platelet activity, tissue repair, and signaling pathways, were differentially affected by BBBD, with a strong dependence on the anesthetic. We conclude that the underlying transcriptomic response to FUS-mediated BBBD may be powerfully influenced by anesthesia. These findings raise considerations for the translation of FUS-BBBD delivery approaches that impact, in particular, metabolism, tissue repair, and intracellular signaling.
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