ArticleBMC plant biology2021
Characterization of full-length transcriptome in Saccharum officinarum and molecular insights into tiller development.
Article in BMC plant biology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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13 citing papers in PubMed, 27 citations in OpenAlex.
- Systematic analysis of the ShRZFP gene family and its association with tiller regulation in sugarcane.BMC plant biology · 2026Article
- Integrated multi-omics analysis reveals hormonal and nutrient networks regulating sugarcane tillering.Frontiers in plant science · 2026Article
- Directly replanting with GF296 extends the years of ratooning and yield in sugarcane.Scientific reports · 2025Article
- Genetic and molecular insights into tiller development and approaches for crop yield improvement.Frontiers in plant science · 2025Review
- Identification of functional pathways and hub genes associated with the heterochronic development of sugarcane axillary buds and sett roots through multi-omics analysis.Frontiers in plant science · 2025Article
- Tandem mass tag (TMT)-based quantitative proteomics analysis reveals the different responses of contrasting alfalfa varieties to drought stress.BMC genomics · 2024Article
- Update on functional analysis of long non-coding RNAs in common crops.Frontiers in plant science · 2024Review
- Theory to practice: a success in breeding sugarcane variety YZ08-1609 known as the King of Sugar.Frontiers in plant science · 2024Article
- The subgenome Saccharum spontaneum contributes to sugar accumulation in sugarcane as revealed by full-length transcriptomic analysis.Journal of advanced research · 2023Article
- Screening and identification of multiple abiotic stress responsive candidate genes based on hybrid-sequencing inHeliyon · 2023Article
- Review
- Full-length transcriptome sequencing reveals the molecular mechanism of potato seedlings responding to low-temperature.BMC plant biology · 2022Article
- Chromosome-scale assembly and population diversity analyses provide insights into the evolution of Sapindus mukorossi.Horticulture research · 2022Article
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Authors and funding
12 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundAlthough extensive breeding efforts are ongoing in sugarcane (Saccharum officinarum L.), the average yield is far below the theoretical potential. Tillering is an important component of sugarcane yield, however, the molecular mechanism underlying tiller development is still elusive. The limited genomic data in sugarcane, particularly due to its complex and large genome, has hindered in-depth molecular studies.
resultsHerein, we generated full-length (FL) transcriptome from developing leaf and tiller bud samples based on PacBio Iso-Seq. In addition, we performed RNA-seq from tiller bud samples at three developmental stages (T0, T1 and T2) to uncover key genes and biological pathways involved in sugarcane tiller development. In total, 30,360 and 20,088 high-quality non-redundant isoforms were identified in leaf and tiller bud samples, respectively, representing 41,109 unique isoforms in sugarcane. Likewise, we identified 1063 and 1037 alternative splicing events identified in leaf and tiller bud samples, respectively. We predicted the presence of coding sequence for 40,343 isoforms, 98% of which was successfully annotated. Comparison with previous FL transcriptomes in sugarcane revealed 2963 unreported isoforms. In addition, we characterized 14,946 SSRs from 11,700 transcripts and 310 lncRNAs. By integrating RNA-seq with the FL transcriptome, 468 and 57 differentially expressed genes (DEG) were identified in T1vsT0 and T2vsT0, respectively. Strong up-regulation of several pyruvate phosphate dikinase and phosphoenolpyruvate carboxylase genes suggests enhanced carbon fixation and protein synthesis to facilitate tiller growth. Similarly, up-regulation of linoleate 9S-lipoxygenase and lipoxygenase genes in the linoleic acid metabolism pathway suggests high synthesis of key oxylipins involved in tiller growth and development.
conclusionsCollectively, we have enriched the genomic data available in sugarcane and provided candidate genes for manipulating tiller formation and development, towards productivity enhancement in sugarcane.
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