ArticleBiosensors & bioelectronics2021
Rapid and highly sensitive one-tube colorimetric RT-LAMP assay for visual detection of SARS-CoV-2 RNA.
Article in Biosensors & bioelectronics, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 60 citations in OpenAlex.
- AI-Driven Workflow for Automated Colorimetric Isothermal Amplification for Robust Pathogen Detection on Portable Devices.ACS omega · 2026Article
- CRISPR/Cas12a with Universal crRNA for Indiscriminate Virus Detection.Molecules (Basel, Switzerland) · 2024Article
- The Role and Value of Professional Rapid Testing of Acute Respiratory Infections (ARIs) in Europe: A Special Focus on the Czech Republic, Poland, and Romania.Diagnostics (Basel, Switzerland) · 2024Review
- β-Cyclodextrin-Stabilized Silver Nanoparticle Production Combined with Loop-Mediated Isothermal Amplification for the Visual Detection of Contagious Pathogens.Micromachines · 2024Article
- Ultrasensitive and fast detection of SARS-CoV-2 using RT-LAMP without pH-dependent dye.Functional & integrative genomics · 2024Article
- Palm Multidiagnostic ofTransboundary and emerging diseases · 2024Article
- Swift and portable detection ofFrontiers in microbiology · 2024Article
- Rapid Detection ofJournal of microbiology and biotechnology · 2023Article
- An ultrasensitive aptasensor of SARS-CoV-2 N protein based on ion current rectification with nanopipettes.Sensors and actuators. B, Chemical · 2023Article
- CRISPR-Cas12a coupled with universal gold nanoparticle strand-displacement probe for rapid and sensitive visual SARS-CoV-2 detection.Sensors and actuators. B, Chemical · 2023Article
- Rapid Onsite Visual Detection of Orf Virus Using a Recombinase-Aided Amplification Assay.Life (Basel, Switzerland) · 2023Article
- Integrated microdroplet array platform with temperature controller and micro-stirring for ultra-fast SARS-CoV-2 detection.Biosensors & bioelectronics · 2023Article
- Direct capture and amplification of nucleic acids using a universal, elution-free magnetic bead-based method for rapid pathogen detection in multiple types of biological samples.Analytical and bioanalytical chemistry · 2023Article
- Review
- Rapid Detection of Attomolar SARS-CoV-2 Nucleic Acids in All-Dielectric Metasurface Biosensors.Biosensors · 2022Article
- Advances in Biosensing Technologies for Diagnosis of COVID-19.Biosensors · 2022Review
- Toward a Home Test for COVID-19 Diagnosis: DNA Machine for Amplification-Free SARS-CoV-2 Detection in Clinical Samples.ChemMedChem · 2022Article
- Editorial on COVID-19 biosensing technologies- 2d Edition.Biosensors & bioelectronics · 2022Article
- Comparative Evaluation of Different Surface Coatings of FeInternational journal of molecular sciences · 2022Article
- Paper-based netlike rolling circle amplification (NRCA) for ultrasensitive and visual detection of SARS-CoV-2.Sensors and actuators. B, Chemical · 2022Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Coronavirus disease 2019 (COVID-19), caused by SARS-CoV-2 (severe acute respiratory syndrome coronavirus 2) is a highly contagious disease. To tame the continuously raging outbreak of COVID-19, developing a cheap, rapid and sensitive testing assay is absolutely imperative. Herein, we developed a one-tube colorimetric RT-LAMP assay for the visual detection of SARS-CoV-2 RNA. The assay integrates Si-OH magnetic beads (MBs)-based fast RNA extraction and rapid isothermal amplification in a single tube, thus bypassing the RNA elution step and directly amplifying on-beads RNA molecules with the visualized results. This one-tube assay has a limit of detection (LOD) as low as 200 copies/mL for sample input volumes of up to 600 μL, and can be performed in less than 1 h from sample collection to result readout. This assay demonstrated a 100% concordance with the gold standard test RT-qPCR test by using 29 clinical specimens and showed high specificity. This one-tube colorimetric RT-LAMP assay can serve as an alternative platform for a rapid and sensitive diagnostic test for COVID-19 and is particularly suitable for use at community clinics or township hospitals.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.