ArticleGenetics2021
In vivo tissue-specific chromatin profiling in Drosophila melanogaster using GFP-tagged nuclei.
Article in Genetics, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 21 citations in OpenAlex.
- The Drosophila SWI/SNF complex subunit Bap60 is required for training-induced gene transcription during long-term memory formation.Genetics · 2026Article
- Increased Glycine-N-methyltransferase expression disrupts light-dependent gene expression rhythms in the Drosophila eye.Journal of cell science · 2026Article
- Broad Epigenetic Shifts in the Aging Drosophila Retina Contribute to Its Altered Diurnal Rhythmic Transcriptome.Aging cell · 2026Article
- Article
- Eye proteome of Drosophila melanogaster.Proteomics · 2024Article
- Transcriptional changes in specific subsets of Drosophila neurons following inhibition of the serotonin transporter.Translational psychiatry · 2023Article
- Establishing the contribution of active histone methylation marks to the aging transcriptional landscape of Drosophila photoreceptors.Scientific reports · 2023Article
- Transcriptional changes in specific subsets of Drosophila neurons following inhibition of the serotonin transporter.Research square · 2023Article
- Targeted RNAi screen identifies transcriptional mechanisms that prevent premature degeneration of adult photoreceptors.Frontiers in epigenetics and epigenomics · 2023Article
- Tissue-Specific, Genome-wide Mapping of R-loops inBio-protocol · 2022Article
- Optimized assay for transposase-accessible chromatin by sequencing (ATAC-seq) library preparation from adult Drosophila melanogaster neurons.Scientific reports · 2022Article
- Proper control of R-loop homeostasis is required for maintenance of gene expression and neuronal function during aging.Aging cell · 2022Article
- Aging and Light Stress Result in Overlapping and Unique Gene Expression Changes in Photoreceptors.Genes · 2022Article
- Chiffon triggers global histone H3 acetylation and expression of developmental genes in Drosophila embryos.Journal of cell science · 2022Article
- The Clock:Cycle complex is a major transcriptional regulator of Drosophila photoreceptors that protects the eye from retinal degeneration and oxidative stress.PLoS genetics · 2022Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
The chromatin landscape defines cellular identity in multicellular organisms with unique patterns of DNA accessibility and histone marks decorating the genome of each cell type. Thus, profiling the chromatin state of different cell types in an intact organism under disease or physiological conditions can provide insight into how chromatin regulates cell homeostasis in vivo. To overcome the many challenges associated with characterizing chromatin state in specific cell types, we developed an improved approach to isolate Drosophila melanogaster nuclei tagged with a GFPKASH protein. The perinuclear space-localized KASH domain anchors GFP to the outer nuclear membrane, and expression of UAS-GFPKASH can be controlled by tissue-specific Gal4 drivers. Using this protocol, we profiled chromatin accessibility using an improved version of Assay for Transposable Accessible Chromatin followed by sequencing (ATAC-seq), called Omni-ATAC. In addition, we examined the distribution of histone marks using Chromatin immunoprecipitation followed by sequencing (ChIP-seq) and Cleavage Under Targets and Tagmentation (CUT&Tag) in adult photoreceptor neurons. We show that the chromatin landscape of photoreceptors reflects the transcriptional state of these cells, demonstrating the quality and reproducibility of our approach for profiling the transcriptome and epigenome of specific cell types in Drosophila.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.