Evidence map›Paper›PMID 34021482›Full record

ArticleMolecular neurobiology2021

Insight into Glutamatergic Involvement in Rewarding Effects of Mephedrone in Rats: In Vivo and Ex Vivo Study.

Olga Wronikowska, Maria Zykubek, Agnieszka Michalak, Anna Pankowska, Paulina Kozioł, Anna Boguszewska-Czubara, Łukasz Kurach, Artur Łazorczyk, Katarzyna Kochalska, Sylwia Talarek and 4 more

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Molecular neurobiology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
0.8field-weighted citation impact, top 33% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 9 citations in OpenAlex.

  1. Article
  2. Mephedrone and Its Metabolites: A Narrative Review.International journal of molecular sciences · 2025
    Review
  3. Review
  4. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors at 1 institution in 1 country.

Olga WronikowskaDepartment of Medical Chemistry, Medical University of Lublin, Chodzki 4a Street, 20-093, Lublin, Poland.
Maria ZykubekIndependent Laboratory of Behavioral Studies, Chair and Department of Medical Chemistry, Medical University of Lublin, Chodzki 4a Street, 20-093, Lublin, Poland.
Agnieszka MichalakIndependent Laboratory of Behavioral Studies, Chair and Department of Medical Chemistry, Medical University of Lublin, Chodzki 4a Street, 20-093, Lublin, Poland.
Anna PankowskaDepartment of Radiography, Medical University of Lublin, Staszica 16 Street, 20-081, Lublin, Poland.
Paulina KoziołDepartment of Radiography, Medical University of Lublin, Staszica 16 Street, 20-081, Lublin, Poland.
Anna Boguszewska-CzubaraDepartment of Medical Chemistry, Medical University of Lublin, Chodzki 4a Street, 20-093, Lublin, Poland.
Łukasz KurachIndependent Laboratory of Behavioral Studies, Chair and Department of Medical Chemistry, Medical University of Lublin, Chodzki 4a Street, 20-093, Lublin, Poland.
Artur ŁazorczykDepartment of Radiography, Medical University of Lublin, Staszica 16 Street, 20-081, Lublin, Poland.
Katarzyna KochalskaDepartment of Radiography, Medical University of Lublin, Staszica 16 Street, 20-081, Lublin, Poland.
Sylwia TalarekDepartment of Pharmacology and Pharmacodynamics, Medical University of Lublin, Chodzki 4a Street, 20-093, Lublin, Poland.
Tymoteusz SłowikCentre of Experimental Medicine, Medical University of Lublin, Jaczewskiego 8 Street, 20-090, Lublin, Poland.
Radosław PieturaDepartment of Radiography, Medical University of Lublin, Staszica 16 Street, 20-081, Lublin, Poland.
Joanna KurzepaI Department of Medical Radiology, Medical University of Lublin, Jaczewskiego 8 Street, 20-090, Lublin, Poland.
Barbara BudzyńskaIndependent Laboratory of Behavioral Studies, Chair and Department of Medical Chemistry, Medical University of Lublin, Chodzki 4a Street, 20-093, Lublin, Poland. barbarabudzynska@umlub.pl.ORCID http://orcid.org/0000-0001-5917-9285
Medical University of Lublin · PL

Funding

Narodowe Centrum Nauki 2017/25/B/NZ7/02410
6 · The paper itself

Abstract

Mephedrone is a widely used drug of abuse, exerting its effects by interacting with monoamine transporters. Although this mechanism has been widely studied heretofore, little is known about the involvement of glutamatergic transmission in mephedrone effects. In this study, we comprehensively evaluated glutamatergic involvement in rewarding effects of mephedrone using an interdisciplinary approach including (1) behavioural study on effects of memantine (non-selective NMDA antagonist) on expression of mephedrone-induced conditioned place preference (CPP) in rats; (2) evaluation of glutamate concentrations in the hippocampus of rats following 6 days of mephedrone administration, using in vivo magnetic resonance spectroscopy (MRS); and (3) determination of glutamate levels in the hippocampus of rats treated with mephedrone and subjected to MRS, using ion-exchange chromatography. In the presented research, we confirmed priorly reported mephedrone-induced rewarding effects in the CPP paradigm and showed that memantine (5 mg/kg) was able to reverse the expression of this effect. MRS study showed that subchronic mephedrone administration increased glutamate level in the hippocampus when measured in vivo 24 h (5 mg/kg, 10 mg/kg and 20 mg/kg) and 2 weeks (5 mg/kg and 20 mg/kg) after last injection. Ex vivo chromatographic analysis did not show significant changes in hippocampal glutamate concentrations; however, it showed similar results as obtained in the MRS study proving its validity. Taken together, the presented study provides new insight into glutamatergic involvement in rewarding properties of mephedrone.

Indexed as

RewardAnimalsExcitatory Amino Acid AntagonistsHippocampusMagnetic Resonance SpectroscopyMaleMethamphetamineMotor ActivityRatsRats, WistarReceptors, N-Methyl-D-AspartateExcitatory Amino Acid AntagonistsmephedroneMethamphetamineReceptors, N-Methyl-D-AspartateChromatographyGlutamateMephedroneMRSPlace preference

Identifiers

PMID34021482
PMCPMC8487417
OpenAlexW3162465290

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.