ArticleNature communications2021
Co-development of central and peripheral neurons with trunk mesendoderm in human elongating multi-lineage organized gastruloids.
Article in Nature communications, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 49 papers.
What it found
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Who cites it
49 citing papers in PubMed, 85 citations in OpenAlex.
- Investigation of embryogenesis in a dish: the development and application of gastruloid models from pluripotent stem cells.Cell & bioscience · 2026Review
- Pleiotropic germline PTEN mutations influence gastrulation through dysregulated AKT activation.NPJ genomic medicine · 2026Article
- Association Between Head Circumference Growth and Peripheral Nerve Cross-Sectional Area Growth in Infants: A Potential Future Biomarker for Central and Peripheral Nerve Maturation.Neuropediatrics · 2026Article
- Progress in stem cell-based embryo models and their applications in developmental biology and biomedicine.Nature reviews. Molecular cell biology · 2026Review
- Organoid Technology as a Promising Frontier in Organ Transplantation and Regenerative Medicine.International journal of stem cells · 2026Review
- Organizers in a dish: Modeling human CNS morphogenesis.Developmental cell · 2026Review
- Human Organoids Reveal PTEN-Driven Mesendoderm Specification via Retinoic Acid Signaling Suppression.Research (Washington, D.C.) · 2026Article
- Spinal motor neuron degeneration after brachial plexus avulsion: mechanisms and therapeutic targets.Frontiers in neuroscience · 2026Review
- Peripheral Nerve-Cancer Interactions in the Tumor Microenvironment: A Three-Dimensional Framework Integrating Mechanisms, Modulators, and Therapeutic Strategies.Research (Washington, D.C.) · 2026Review
- Technologies and applications of current human cardiac organoids.Acta pharmaceutica Sinica. B · 2025Review
- Artificial Uterus and Artificial Embryos: Unsolved Tasks.Reproductive sciences (Thousand Oaks, Calif.) · 2025Review
- Stem cell-based human embryo models: current knowledge and open questions.Stem cell research & therapy · 2025Review
- Generation of Neural Organoids and Their Application in Disease Modeling and Regenerative Medicine.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Review
- Advances in engineered models of peri-gastrulation.iScience · 2025Review
- Pluripotent cell states and fates in human embryo models.Development (Cambridge, England) · 2025Review
- Article
- Timely TGFβ signalling inhibition induces notochord.Nature · 2025Article
- In vitro modelling of anterior primitive streak patterning with human pluripotent stem cells identifies the path to notochord progenitors.Development (Cambridge, England) · 2024Article
- Three-dimensional stem cell models of mammalian gastrulation.BioEssays : news and reviews in molecular, cellular and developmental biology · 2024Review
- Generation of self-renewing neuromesodermal progenitors with neuronal and skeletal muscle bipotential from human embryonic stem cells.Cell reports methods · 2024Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Stem cell technologies including self-assembling 3D tissue models provide access to early human neurodevelopment and fundamental insights into neuropathologies. Gastruloid models have not been used to investigate co-developing central and peripheral neuronal systems with trunk mesendoderm which we achieve here in elongating multi-lineage organized (EMLO) gastruloids. We evaluate EMLOs over a forty-day period, applying immunofluorescence of multi-lineage and functional biomarkers, including day 16 single-cell RNA-Seq, and evaluation of ectodermal and non-ectodermal neural crest cells (NCCs). We identify NCCs that differentiate to form peripheral neurons integrated with an upstream spinal cord region after day 8. This follows initial EMLO polarization events that coordinate with endoderm differentiation and primitive gut tube formation during multicellular spatial reorganization. This combined human central-peripheral nervous system model of early organogenesis highlights developmental events of mesendoderm and neuromuscular trunk regions and enables systemic studies of tissue interactions and innervation of neuromuscular, enteric and cardiac relevance.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.