Evidence map›Paper›PMID 34020639›Full record

ArticleCancer cell international2021

Circ_0000745 strengthens the expression of CCND1 by functioning as miR-488 sponge and interacting with HuR binding protein to facilitate the development of oral squamous cell carcinoma.

Kuangzheng Li, Xiaosheng Fan, Ziyi Yan, Jia Zhan, Fangyun Cao, Yixia Jiang

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cancer cell international, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
9citing papers in PubMed, 1 pooled it
1.4field-weighted citation impact, top 19% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

9 citing papers in PubMed, 1 synthesis or guideline pooled it, 18 citations in OpenAlex.

  1. Pooled it
  2. AFAP1-AS1 regulates oral squamous cell carcinoma development through the miR-93-3p/CCND1 signaling pathway.Mammalian genome : official journal of the International Mammalian Genome Society · 2026
    Article
  3. Review
  4. Circular RNAs in Cell Cycle Regulation of Cancers.International journal of molecular sciences · 2024
    Review
  5. Review
  6. Review
  7. Review
  8. Article
  9. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 1 institution in 1 country.

Kuangzheng LiDepartment of Oral and Maxillofacial Surgery, The Second Affiliated Hospital, University of South China, Hengyang City, Hunan Province, China.
Xiaosheng FanDepartment of Oral and Maxillofacial Surgery, The Second Affiliated Hospital, University of South China, Hengyang City, Hunan Province, China.
Ziyi YanDepartment of Oral and Maxillofacial Surgery, The Second Affiliated Hospital, University of South China, Hengyang City, Hunan Province, China.
Jia ZhanDepartment of Oral and Maxillofacial Surgery, The Second Affiliated Hospital, University of South China, Hengyang City, Hunan Province, China.
Fangyun CaoDepartment of Oral and Maxillofacial Surgery, The Second Affiliated Hospital, University of South China, Hengyang City, Hunan Province, China.
Yixia JiangDepartment of Respiration, The Second Affiliated Hospital, University of South China, No. 35 Jiefang Avenue, Zhengxiang District, Hengyang City, Hunan Province, 421000, People's Republic of China. 2011001895@usc.edu.cn.ORCID http://orcid.org/0000-0002-5727-8535
University of South China · CN

Funding

Expression and significance of CD117 in salivary gland tumors 2019jh011104
6 · The paper itself

Abstract

backgroundThe implication of circular RNAs (circRNAs) in human cancers has aroused much concern. In this study, we investigated the function of circ_0000745 and its potential functional mechanisms in oral squamous cell carcinoma (OSCC) to further understand OSCC pathogenesis.

methodsThe expression of circ_0000745, miR-488 and cyclin D1 (CCND1) mRNA was measured by quantitative real-time polymerase chain reaction (qPCR). Cell proliferation capacity was assessed by cell counting kit-8 (CCK-8) assay and colony formation assay. Cell cycle progression and cell apoptosis were determined by flow cytometry assay. The protein levels of CCND1, PCNA, Cleaved-caspase 3 and HuR were detected by western blot. Animal study was conducted to identify the role of circ_0000745 in vivo. The targeted relationship was verified by dual-luciferase reporter assay, pull-down assay or RNA immunoprecipitation (RIP) assay.

resultsThe expression of circ_0000745 was increased in OSCC tissues and cells. Circ_0000745 downregulation inhibited OSCC cell proliferation and induced cell cycle arrest and apoptosis in vitro, as well as blocked tumor growth in vivo. MiR-488 was a target of circ_0000745, and circ_0000745 downregulation suppressed OSCC development by enriching miR-488. Besides, circ_0000745 regulated CCND1 expression by targeting miR-488. In addition, circ_0000745 regulated CCND1 expression by interacting with HuR protein. CCND1 knockdown also inhibited OSCC cell proliferation and induced cell cycle arrest and apoptosis in vitro, and CCND1 overexpression recovered the inhibitory effects on OSCC cell malignant behaviors caused by circ_0000745 downregulation.

conclusionsCirc_0000745 regulated the expression of CCND1 partly by acting as miR-488 sponge and interacting with HuR protein, thus promoting the progression of OSCC.

Indexed as

CCND1Circ_0000745HuRmiR-488OSCC

Identifiers

PMID34020639
PMCPMC8139082
OpenAlexW3160584943

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.