Evidence map›Paper›PMID 34019636›Full record

ArticleNucleic acids research2021

pLannotate: engineered plasmid annotation.

Matthew J McGuffie, Jeffrey E Barrick

Open access · goldAbstract read
In one paragraph

Article in Nucleic acids research, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.

0numbers the graph read from it
0cells of the map it votes in
37citing papers in PubMed
3.9field-weighted citation impact, top 5% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

37 citing papers in PubMed, 62 citations in OpenAlex.

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  14. Genomic characterization of a multidrug-resistantFrontiers in microbiology · 2025
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  17. Modular DNA Construct Design for High-Throughput Golden Gate Assembly.Methods in molecular biology (Clifton, N.J.) · 2025
    Article
  18. Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors at 1 institution in 1 country.

Matthew J McGuffieDepartment of Molecular Biosciences, Center for Systems and Synthetic Biology, The University of Texas at Austin, 2500 Speedway A5000, Austin, TX 78712, USA.
Jeffrey E BarrickDepartment of Molecular Biosciences, Center for Systems and Synthetic Biology, The University of Texas at Austin, 2500 Speedway A5000, Austin, TX 78712, USA.ORCID 0000-0003-0888-7358
The University of Texas at Austin · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Engineered plasmids are widely used in the biological sciences. Since many plasmids contain DNA sequences that have been reused and remixed by researchers for decades, annotation of their functional elements is often incomplete. Missing information about the presence, location, or precise identity of a plasmid feature can lead to unintended consequences or failed experiments. Many engineered plasmids contain sequences-such as recombinant DNA from all domains of life, wholly synthetic DNA sequences, and engineered gene expression elements-that are not predicted by microbial genome annotation pipelines. Existing plasmid annotation tools have limited feature libraries and do not detect incomplete fragments of features that are present in many plasmids for historical reasons and may impact their newly designed functions. We created the open source pLannotate web server so users can quickly and comprehensively annotate plasmid features. pLannotate is powered by large databases of genetic parts and proteins. It employs a filtering algorithm to display only the most relevant feature matches and also reports feature fragments. Finally, pLannotate displays a graphical map of the annotated plasmid, explains the provenance of each feature prediction, and allows results to be downloaded in a variety of formats. The webserver for pLannotate is accessible at: http://plannotate.barricklab.org/.

Indexed as

Molecular Sequence AnnotationSoftwareAmino Acyl-tRNA SynthetasesBioengineeringDatabases, GeneticDependovirusInternetPlasmidsAmino Acyl-tRNA Synthetases

Identifiers

PMID34019636
PMCPMC8262757
OpenAlexW3163366850

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.