ArticleEmerging microbes & infections2021
Multiple detection and spread of novel strains of the SARS-CoV-2 B.1.177 (B.1.177.75) lineage that test negative by a commercially available nucleocapsid gene real-time RT-PCR.
Article in Emerging microbes & infections, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed.
- Detection of community-acquired respiratory viruses during COVID-19 pandemic in subtropical region in Japan.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2024Article
- Digital PCR Discriminates between SARS-CoV-2 Omicron Variants and Immune Escape Mutations.Microbiology spectrum · 2023Article
- One health system supporting surveillance during COVID-19 epidemic in Abruzzo region, southern Italy.One health (Amsterdam, Netherlands) · 2023Article
- Impact of G29179T mutation on two commercial PCR assays for SARS-CoV-2 detection.Journal of virological methods · 2023Article
- Variant-specific RT-qPCR for rapid screening of B.1.617 mutations in SARS-CoV-2.The Libyan journal of medicine · 2022Article
- A Deletion Encompassing the Furin Cleavage Site in the Spike Encoding Gene Does Not Alter SARS-CoV-2 Replication in Lung Tissues of Mink and Neutralization by Convalescent Human Serum Samples.Pathogens (Basel, Switzerland) · 2022Article
- Epidemiological and genomic findings of the first documented Italian outbreak of SARS-CoV-2 Alpha variant of concern.Epidemics · 2022Article
- Impact of SARS-CoV-2 Variants on the Analytical Sensitivity of rRT-PCR Assays.Journal of clinical microbiology · 2022Article
- Human SARS-CoV-2 has evolved to increase U content and reduce genome size.International journal of biological macromolecules · 2022Article
- Unusual N Gene Dropout and Ct Value Shift in Commercial Multiplex PCR Assays Caused by Mutated SARS-CoV-2 Strain.Diagnostics (Basel, Switzerland) · 2022Article
- A deletion in the N gene of SARS-CoV-2 may reduce test sensitivity for detection of SARS-CoV-2.Diagnostic microbiology and infectious disease · 2022Article
- Article
- In vitro evaluation of the effect of mutations in primer binding sites on detection of SARS-CoV-2 by RT-qPCR.Journal of virological methods · 2022Article
- Whole-Genome Sequences of SARS-CoV-2 Lineage B.1.525 Strains (Variant η) Detected from Patients in the Abruzzo Region (Central Italy) during Spring 2021.Microbiology resource announcements · 2021Article
- Limitation of Screening of Different Variants of SARS-CoV-2 by RT-PCR.Diagnostics (Basel, Switzerland) · 2021Article
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14 authors.
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Abstract
Several lineages of SARS-CoV-2 are currently circulating worldwide. During SARS-CoV-2 diagnostic activities performed in Abruzzo region (central Italy) several strains belonging to the B.1.177.75 lineage tested negative for the N gene but positive for the ORF1ab and S genes (+/+/- pattern) by the TaqPath COVID-19 CE-IVD RT-PCR Kit manufactured by Thermofisher. By sequencing, a unique mutation, synonymous 28948C > T, was found in the N-negative B.1.177.75 strains. Although we do not have any knowledge upon the nucleotide sequences of the primers and probe adopted by this kit, it is likely that N gene dropout only occurs when 28948C > T is coupled with 28932C > T, this latter present, in turn, in all B.1.177.75 sequences available on public databases. Furthermore, epidemiological analysis was also performed. The majority of the N-negative B.1.177.75 cases belonged to two clusters apparently unrelated to each other and both clusters involved young people. However, the phylogeny for sequences containing the +/+/- pattern strongly supports a genetic connection and one common source for both clusters. Though, genetic comparison suggests a connection rather than indicating the independent emergence of the same mutation in two apparently unrelated clusters. This study highlights once more the importance of sharing genomic data to link apparently unrelated epidemiological clusters and to, remarkably, update molecular tests.
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