Evidence map›Paper›PMID 34019292›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2021

Methods for Determining Myc-Induced Apoptosis.

Dan Lu, Catherine Wilson, Trevor D Littlewood

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Dan LuDepartment of Biochemistry, University of Cambridge, Cambridge, UK.
Catherine WilsonDepartment of Pharmacology, University of Cambridge, Cambridge, UK.
Trevor D LittlewoodDepartment of Biochemistry, University of Cambridge, Cambridge, UK. tdl2@cam.ac.uk.

Funding

Cancer Research UK 19013
6 · The paper itself

Abstract

Although many oncoproteins promote cell growth and proliferation, some also possess the potential to induce cell cycle arrest or cell death by apoptosis. Elevated and deregulated expression of the Myc protein promotes apoptosis in both cultured cells and in some tissues in vivo. Here we describe techniques to detect Myc-induced apoptosis in vitro using flow cytometry, microscopy, and immunoblotting, and in vivo using immunohistochemical staining, immunoblotting, and analysis of RNA expression.

Indexed as

AnimalsAnnexin A5ApoptosisCell CycleCell Cycle CheckpointsCell DeathCell ProliferationDNAFlow CytometryGenes, mycHumansImmunohistochemistryIn Situ Nick-End LabelingMiceProto-Oncogene Proteins c-mycAnnexin A5DNAProto-Oncogene Proteins c-mycAnnexin VApoptosisCleaved caspase 3Flow cytometryHematoxylin and eosinImmunohistochemistryMicroscopyRNA expressionTUNEL

Identifiers

PMID34019292

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.