ArticlePlant reproduction2021
A dynamic intron retention program regulates the expression of several hundred genes during pollen meiosis.
Article in Plant reproduction, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 23 citations in OpenAlex.
- Pan-Genomic Dissection of GH1 β-Glucosidases inPlants (Basel, Switzerland) · 2026Article
- Intron Retention: A Reemerging Paradigm in RNA Biology and Post-Transcriptional Gene Regulation.Genes · 2025Review
- Graphical pangenomics-enabled characterization of structural variant impact on gene expression in Brassica napus.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2025Article
- Identifying long non-coding RNAs involved in heat stress response during wheat pollen development.Frontiers in plant science · 2024Article
- Transcriptomics reveals a core transcriptional network of K-type cytoplasmic male sterility microspore abortion in wheat (Triticum aestivum L.).BMC plant biology · 2023Article
- Regulatory dynamics of gene expression in the developing male gametophyte of Arabidopsis.Plant reproduction · 2023Article
- Cell-type-specific alternative splicing in the Arabidopsis germline.Plant physiology · 2023Article
- Transgressive and parental dominant gene expression and cytosine methylation during seed development in Brassica napus hybrids.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2023Article
- Genome-wide analysis reveals the crucial role of lncRNAs in regulating the expression of genes controlling pollen development.Plant cell reports · 2023Article
- Biological Parts for Engineering Abiotic Stress Tolerance in Plants.Biodesign research · 2022Review
- Long Intergenic Noncoding RNA (lincRNA) Discovery from Non-Strand-Specific RNA-Seq Data.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Rapid Transcriptional Reprogramming Associated With Heat Stress-Induced Unfolded Protein Response in DevelopingFrontiers in plant science · 2022Article
- Role of long non-coding RNAs in rice reproductive development.Frontiers in plant science · 2022Review
- Circular RNAs Repertoire and Expression Profile duringInternational journal of molecular sciences · 2021Article
- Downregulation of BarleyFrontiers in plant science · 2021Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
key messageIntron retention is a stage-specific mechanism of functional attenuation of a subset of co-regulated, functionally related genes during early stages of pollen development. To improve our understanding of the gene regulatory mechanisms that drive developmental processes, we performed a genome-wide study of alternative splicing and isoform switching during five key stages of pollen development in field mustard, Brassica rapa. Surprisingly, for several hundred genes (12.3% of the genes analysed), isoform switching results in stage-specific expression of intron-retaining transcripts at the meiotic stage of pollen development. In such cases, we report temporally regulated switching between expression of a canonical, translatable isoform and an intron-retaining transcript that is predicted to produce a truncated and presumably inactive protein. The results suggest a new pervasive mechanism underlying modulation of protein levels in a plant developmental program. The effect is not based on gene expression induction but on the type of transcript produced. We conclude that intron retention is a stage-specific mechanism of functional attenuation of a subset of co-regulated, functionally related genes during meiosis, especially genes related to ribosome biogenesis, mRNA transport and nuclear envelope architecture. We also propose that stage-specific expression of a non-functional isoform of Brassica rapa BrSDG8, a non-redundant member of histone methyltransferase gene family, linked to alternative splicing regulation, may contribute to the intron retention observed.
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