Evidence map›Paper›PMID 34018360›Full record

ArticleSichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition2021

[Osteogenic Capacity and Mettl14 and Notch1 Expression of Adipose-Derived Stem Cells from Osteoporotic Rats].

Mao-Geng Feng, Shuang-Lin Yang, Dao-Wen Luo, Shuang-Lin Peng, Fang-Zhi Lou, Jin-Gang Xiao

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.2field-weighted citation impact, top 48% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 3 citations in OpenAlex.

  1. Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 1 institution in 1 country.

Mao-Geng FengDepartment of Oral and Maxillofacial Surgery, the Affiliated Stomatology Hospital of Southwest Medical University, Luzhou 646000, China.
Shuang-Lin YangOrofacial Reconstruction and Regeneration Laboratory, the Affiliated Stomatology Hospital of Southwest Medical University, Luzhou 646000, China.
Dao-Wen LuoDepartment of Oral and Maxillofacial Surgery, the Affiliated Stomatology Hospital of Southwest Medical University, Luzhou 646000, China.
Shuang-Lin PengOrofacial Reconstruction and Regeneration Laboratory, the Affiliated Stomatology Hospital of Southwest Medical University, Luzhou 646000, China.
Fang-Zhi LouOrofacial Reconstruction and Regeneration Laboratory, the Affiliated Stomatology Hospital of Southwest Medical University, Luzhou 646000, China.
Jin-Gang XiaoDepartment of Oral and Maxillofacial Surgery, the Affiliated Stomatology Hospital of Southwest Medical University, Luzhou 646000, China.
Southwest Medical University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

objectiveTo investigate the differences in the osteogenic capacity of osteoporotic adipose-derived stem cells (OP-ASCs) and normal control adipose-derived stem cells (Ctrl-ASCs), and to examine the expression levels of RNA methyltransferase like 14 (Mettl14) and the Notch signaling molecule 1 (Notch1).

methodsThe osteoporosis (OP) model of SD rats was established with ovariectomy (OVX). Micro-CT, HE staining and Masson staining were performed to identify the successful establishment of the OP model, OP-ASCs and Ctrl-ASCs were isolated and cultured adherently. Then, the three-way differentiation capacity of the adipose-derived stem cells (ASCs) was determined through alizarin red staining, alcian blue staining and oil red O staining and flow cytometry was conducted to examine the surface antigens CD29, CD44, CD90, CD31, CD34, and CD45. Alizarin red staining and comparison of the mRNA and protein expression of Run-related transcription factor 2 (Runx2) were done to explore the differences in osteogenic potential of OP-ASCs and Ctrl-ASCs. Real-time PCR and Western blot were performed to explore the expression differences of Mettl14 and Notch1 at mRNA and protein levels between OP-ASCs and Ctrl-ASCs.

resultsMicro-CT, HE and Masson staining results showed that the number of trabecular bone decreased and the spacing increased in the tibias of the osteoporosis group (OP group) compared with those of the control group (Ctrl group), indicating that the OP model was established successfully. Three-way differentiation and flow cytometry results confirmed the successful isolation and culture of ASCs. After osteogenic induction, alizarin red staining showed that OP-ASCs had fewer number and more scattered distribution of mineralized nodules than Ctrl-ASCs did. The expression of Runx2 in OP-ASCs was lower than that in Ctrl-ASCs (

conclusionThe osteogenic capacity of OP-ASCs was lower compared with that of Ctrl-ASCs, Mettl14 expression of OP-ASCs was decreased compared with that of Ctrl-ASCs, and the Notch signaling pathway was inhibited in OP-ASCs. The study helps build the foundation for further investigation in the specific mechanisms of Mettl14 and Notch1 during osteogenic differentiation of OP-ASCs.

Indexed as

OsteogenesisStem CellsAdipocytesAdipose TissueAnimalsCell DifferentiationCells, CulturedFemaleHumansMethyltransferasesRatsRats, Sprague-DawleyReceptor, Notch1MethyltransferasesMETTL14 protein, humanNOTCH1 protein, humanNotch1 protein, ratReceptor, Notch1Adipose-derived stem cellsMettl14Notch signaling pathwayOsteogenic differentiationOsteoporotic ratRNA methylation

Identifiers

PMID34018360
PMCPMC10409211
OpenAlexW3162906451

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.