ArticleAnalytical and bioanalytical chemistry2021
A strip of lateral flow gene assay using gold nanoparticles for point-of-care diagnosis of African swine fever virus in limited environment.
Article in Analytical and bioanalytical chemistry, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 34 citations in OpenAlex.
- Biosensors for the detection of arthropod-borne veterinary viruses: A comprehensive review.Veterinary and animal science · 2026Review
- A Microfluidic Chip and a Portable Colorimetric Detection Device for the Rapid, Low-Cost, and Accurate Diagnosis of ASFV.Transboundary and emerging diseases · 2026Article
- Review
- Development of a colloidal gold immunochromatographic strip to detect equine infectious anemia virus.Virology journal · 2025Article
- Technologies for detecting biological risk factors in agricultural products and their applications.Current research in food science · 2025Review
- Highly sensitive multiplexed colorimetric lateral flow immunoassay by plasmon-controlled metal-silica isoform nanocomposites: PINs.Nano convergence · 2024Article
- Strategic nucleic acid detection approaches for diagnosing African swine fever (ASF): navigating disease dynamics.Veterinary research · 2024Review
- Review
- Latest Advances in Arbovirus Diagnostics.Microorganisms · 2023Review
- Development of Lateral Flow Test-System for the Immunoassay of Dibutyl Phthalate in Natural Waters.Biosensors · 2022Article
- Article
- Development of a ladder-shape melting temperature isothermal amplification (LMTIA) assay for detection of African swine fever virus (ASFV).Journal of veterinary science · 2022Article
- Non-nucleic acid extraction and ultra-sensitive detection of African swine fever virus via CRISPR/Cas12a.Applied microbiology and biotechnology · 2022Article
- Non-nucleic acid extraction and ultra-sensitive detection of African swine fever virus via CRISPR/Cas12a.Applied microbiology and biotechnology · 2022Article
- Development of a p72 trimer-based colloidal gold strip for detection of antibodies against African swine fever virus.Applied microbiology and biotechnology · 2022Article
- Signal-enhanced visual strand exchange amplification detection of African swine fever virus by the introduction of multimeric G-quadruplex/hemin DNAzyme.Analytical sciences : the international journal of the Japan Society for Analytical Chemistry · 2022Article
- Advances in the use of nanomaterials for nucleic acid detection in point-of-care testing devices: A review.Frontiers in bioengineering and biotechnology · 2022Review
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Recombinase polymerase amplification (RPA) was combined with lateral flow to develop a gold nanoparticles test strip for point-of-care diagnosis of African swine fever virus (ASFV), which is called lateral flow gene assay (LFGA). Common diagnostic techniques, including polymerase chain reaction (PCR) and immunochromatography, are time-consuming and labor-intensive, and generally require costly instruments. For improvement, this assay used tailed primers to produce DNA duplexes with a single-stranded tail at one end which can hybridize with a gold nanoparticle (AuNP)-labeled oligonucleotide detection probe. And then, biotin attached to the other end of the product bound to streptavidin, which previously fixed to the test line. Therefore, there would form a sandwich structure, and gold nanoparticles labeled on the detection probe would show a red band on the test line of strip. With the low reaction temperature (37~42 °C) and short reaction time (30 min), LFGA can specifically identify ASFV in blood samples infected with ASFV and classical swine fever virus (CSFV), and the LOD was 10
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Registered trials
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