Evidence map›Paper›PMID 34017411›Full record

ArticleAmerican journal of translational research2021

Mechanisms of miR-195-5p and FOXK1 in rat xenograft models of non-small cell lung cancer.

Jiguo Niu, Yiwen Wang, Yonghua Hu, Caili Li, Yue Fang

Open access · greenAbstract read
PubMed Full text at PMC
In one paragraph

Article in American journal of translational research, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
0.3field-weighted citation impact, top 50% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 6 citations in OpenAlex.

  1. Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 4 institutions in 1 country.

Jiguo NiuDepartment of Nuclear Medicine, Gansu Provincial Cancer Hospital Lanzhou 730050, Gansu Province, China.
Yiwen WangDepartment of Clinical Laboratory, Gansu Provincial Cancer Hospital Lanzhou 730050, Gansu Province, China.
Yonghua HuGansu University of Chinese Medicine Lanzhou 730000, Gansu Province, China.
Caili LiSchool of Medicine of Northwest Minzu University Lanzhou 730030, Gansu Province, China.
Yue FangGansu Provincial Hospital of TCM Lanzhou 730050, Gansu Province, China.
Gansu Provincial Hospital · CNGansu Provincial Hospital of TCM · CNGansu University of Traditional Chinese Medicine · CNMinzu University of China · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

objectiveTo investigate the roles and mechanisms of miR-195-5p and forkhead box K1 (FOXK1) in rat xenograft models of non-small cell lung cancer (NSCLC).

methodsRat xenograft models of NSCLC were established. Evaluations of morphology of NSCLC cells and levels of Ki67 and P53 were detected by hematoxylin-eosin (HE) staining and immunohistochemistry (IHC), respectively. The miR-195-5p level in NSCLC was measured by quantitative real-time RT-PCR (qRT-PCR), and FOXK1, Bax, Caspase-3 and Bal-2 levels were quantified by Western blot. And the regulatory relation between miR-195-5p and FOXK1 was determined by dual-luciferase reporter (DLR) assay.

resultsHE staining and IHC demonstrated successful establishment of NSCLC models in which miR-195-5p was downregulated and FOXK1 was upregulated. Pearson correlation showed that miR-195-5p and FOXK1 were inversely associated (r=0.551, P=0.012). DLR assay confirmed the targeted regulatory relation between miR-195-5p and FOXK1, and upregulation of miR-195-5p accelerated apoptosis of tumor cells.

conclusionmiR-195-5p is inversely associated with FOXK1 in NSCLC in rats. Upregulation of miR-195-5p suppresses FOXK1 and accelerates apoptosis of tumor cells, which may serve as an therapeutic target for the treatment of NSCLC.

Indexed as

FOXK1mechanismmiR-195-5pNon-small cell lung cancer

Identifiers

PMID34017411
PMCPMC8129418
OpenAlexW3163803517

What OpenQuestion holds

Textmetadata
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.