ArticleNature communications2021
Wide field light-sheet microscopy with lens-axicon controlled two-photon Bessel beam illumination.
Article in Nature communications, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed.
- Deep-learning triage of three-dimensional pathology datasets for comprehensive and efficient pathologist assessments.Nature biomedical engineering · 2026Article
- Tunable Bessel beam two-photon fluorescence microscopy for high-speed volumetric imaging of brain dynamics.eLife · 2026Article
- Bessel beam side lobe suppression via non-degenerate two-photon excitation.Biomedical optics express · 2026Article
- Thermal lensing effects in two-photon light-sheet microscopy.Biomedical optics express · 2026Article
- Tunable Bessel beam two-photon fluorescence microscopy for high-speed volumetric imaging of brain dynamics.bioRxiv : the preprint server for biology · 2026Article
- Compact way to generate guide star for wavefront sensing in Bessel two-photon microscopy.Scientific reports · 2025Article
- Rapid axially scanned and de-scanned line-scan confocal microscopy with a tunable acoustic gradient index of refraction lens for high-speed volumetricNeurophotonics · 2025Article
- Axicons for improved resolution and depth of focus in adaptive optics scanning light ophthalmoscopy.Biomedical optics express · 2025Article
- Imaging 3D cell cultures with optical microscopy.Nature methods · 2025Review
- Efficient generation of Bessel-Gauss attosecond pulse trains via nonadiabatic phase-matched high-order harmonics.Light, science & applications · 2025Article
- Zebrafish fluorescence imaging platform based on Bessel light sheet illumination.Biomedical optics express · 2025Article
- Trans-scale live-imaging of an E5.5 mouse embryo using incubator-type biaxial light-sheet microscopy.Life science alliance · 2025Article
- Article
- Optical sectioning methods in three-dimensional bioimaging.Light, science & applications · 2025Review
- Sidelobe suppressed Bessel beams for one-photon light-sheet microscopy.Biomedical optics express · 2024Article
- Frontiers in artificial intelligence-directed light-sheet microscopy for uncovering biological phenomena and multi-organ imaging.View (Beijing, China) · 2024Article
- A practical guide to light-sheet microscopy for nanoscale imaging: Looking beyond the cell.Journal of applied physics · 2024Article
- descSPIM: an affordable and easy-to-build light-sheet microscope optimized for tissue clearing techniques.Nature communications · 2024Article
- Extending the Depth of Focus of an Infrared Microscope Using a Binary Axicon Fabricated on Barium Fluoride.Micromachines · 2024Article
- Interferometric modulation for generating extended light sheet: improving field of view.Journal of biomedical optics · 2024Article
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Two-photon excitation can lower phototoxicity and improve penetration depth, but its narrow excitation range restricts its applications in light-sheet microscopy. Here, we propose simple illumination optics, a lens-axicon triplet composed of an axicon and two convex lenses, to generate longer extent Bessel beams. This unit can stretch the beam full width at half maximum of 600-1000 μm with less than a 4-μm waist when using a 10× illumination lens. A two-photon excitation digital scanned light-sheet microscope possessing this range of field of view and ~2-3-μm axial resolution is constructed and used to analyze the cellular dynamics over the whole body of medaka fish. We demonstrate long-term time-lapse observations over several days and high-speed recording with ~3 mm
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.