Evidence map›Paper›PMID 34016181›Full record

ArticleStem cell research & therapy2021

Strontium ranelate promotes chondrogenesis through inhibition of the Wnt/β-catenin pathway.

Hao Yu, Yan Liu, Xiangwen Yang, Jiajing He, Fan Zhang, Qun Zhong, Xiaojing Guo

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Stem cell research & therapy, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
36citing papers in PubMed, 1 pooled it
5.6field-weighted citation impact, top 3% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

36 citing papers in PubMed, 1 synthesis or guideline pooled it, 51 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 1 institution in 1 country.

Hao Yu *Department of Prosthodontics, Shanghai Stomatological Hospital, Fudan University, Shanghai, 200001, China.
Yan Liu *Department of Prosthodontics, Shanghai Stomatological Hospital, Fudan University, Shanghai, 200001, China.
Xiangwen YangDepartment of Prosthodontics, Shanghai Stomatological Hospital, Fudan University, Shanghai, 200001, China.
Jiajing HeDepartment of Prosthodontics, Shanghai Stomatological Hospital, Fudan University, Shanghai, 200001, China.
Fan ZhangShanghai Key Laboratory of Craniomaxillofacial Development and Diseases, Fudan University, Shanghai, 200001, China.
Qun ZhongDepartment of Prosthodontics, Shanghai Stomatological Hospital, Fudan University, Shanghai, 200001, China.
Xiaojing GuoDepartment of Prosthodontics, Shanghai Stomatological Hospital, Fudan University, Shanghai, 200001, China. guo_xiaojing@fudan.edu.cn.ORCID 0000-0002-6516-0019
Fudan University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundCartilage regeneration is a key step in functional reconstruction for temporomandibular joint osteoarthritis (TMJ-OA) but is a difficult issue to address. Strontium ranelate (SrR) is an antiosteoporosis drug that has been proven to affect OA in recent years, but its effect on chondrogenesis and the underlying mechanism are still unclear.

methodsBone mesenchymal stem cells (BMSCs) from Sprague-Dawley (SD) rats were induced in chondrogenic differentiation medium with or without SrR, XAV-939, and LiCl. CCK-8 assays were used to examine cell proliferation, and alcian blue staining, toluidine blue staining, immunofluorescence, and PCR analysis were performed. Western blot (WB) analyses were used to assess chondrogenic differentiation of the cells. For an in vivo study, 30 male SD rats with cartilage defects on both femoral condyles were used. The defect sites were not filled, filled with silica nanosphere plus gelatine-methacryloyl (GelMA), or filled with SrR-loaded silica nanosphere plus GelMA. After 3 months of healing, paraffin sections were made, and toluidine blue staining, safranin O/fast green staining, and immunofluorescent or immunohistochemical staining were performed for histological evaluation. The data were analyzed by SPSS 26.0 software.

resultsLow concentrations of SrR did not inhibit cell proliferation, and the cells treated with SrR (0.25 mmol/L) showed stronger chondrogenesis than the control. XAV-939, an inhibitor of β-catenin, significantly promoted chondrogenesis, and SrR did not suppress this effect, while LiCl, an agonist of β-catenin, strongly suppressed chondrogenesis, and SrR reversed this inhibitory effect. In vivo study showed a significantly better cartilage regeneration and a lower activation level of β-catenin by SrR-loaded GelMA than the other treatments.

conclusionSrR could promote BMSCs chondrogenic differentiation by inhibiting the Wnt/β-catenin signaling pathway and accelerate cartilage regeneration in rat femoral condyle defects.

Indexed as

ChondrogenesisWnt Signaling PathwayAnimalsbeta CateninCell DifferentiationCells, CulturedMaleRatsRats, Sprague-DawleyThiophenesbeta Cateninstrontium ranelateThiophenesBMSCsCartilage regenerationChondrogenesisStrontium ranelateWnt/β-catenin pathway

Identifiers

PMID34016181
PMCPMC8139050
OpenAlexW3146599428

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.