ArticleReproductive biology and endocrinology : RB&E2021
TMT-based proteomic and bioinformatic analyses of human granulosa cells from obese and normal-weight female subjects.
Article in Reproductive biology and endocrinology : RB&E, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 27 citations in OpenAlex.
- Association of Lifestyle-Induced Weight Loss With Gene Expression in Subcutaneous Adipose Tissue in Metabolic Syndrome.Journal of diabetes · 2025Trial
- Inflammation and polyendocrine metabolic ovarian syndrome: utilizing proteomics to unravel this link in adolescents.Journal of the Endocrine Society · 2026Article
- Network Pharmacology and In Vivo Evaluation of Modified Huanglian Wendan Decoction in Polycystic Ovary Syndrome With Insulin Resistance: Association With AGE-RAGE-Related Signaling.Chemistry & biodiversity · 2026Article
- Overweight/Obesity and Hyperinsulinemia Impair Oocyte Developmental Competence via Cumulus Cell Dysfunction and Oxidative Stress-Mediated Follicular Microenvironment Remodeling.International journal of molecular sciences · 2026Article
- Agomelatine alleviates palmitic acid-induced mouse oocyte meiosis defects by restoring mitochondrial function.International journal of obesity (2005) · 2025Article
- Role of Granulosa Cell Dysfunction in Women Infertility Associated with Polycystic Ovary Syndrome and Obesity.Biomolecules · 2025Review
- The Impact of BMI on PCOS Patients and Transcriptome Profiling and Bioinformatic Analysis of Granulosa Cells in PCOS Patients with High and Low BMI.Reproductive sciences (Thousand Oaks, Calif.) · 2025Article
- Impact of obesity on follicular fluid lipid composition and IVF/ICSI outcomes in Korean women: A lipidomic study.PloS one · 2025Article
- Oxidative stress and energy metabolism abnormalities in polycystic ovary syndrome: from mechanisms to therapeutic strategies.Reproductive biology and endocrinology : RB&E · 2024Review
- Article
- Leptin signalling regulates transcriptional differences in granulosa cells from genetically obese mice but not the activation of NLRP3 inflammasome.Scientific reports · 2024Article
- Proteomic Analysis Identifies Distinct Protein Patterns for High Ovulation inAnimals : an open access journal from MDPI · 2023Article
- Analysis of the quality, accuracy, and readability of patient information on polycystic ovarian syndrome (PCOS) on the internet available in English: a cross-sectional study.Reproductive biology and endocrinology : RB&E · 2023Article
- Prediction of Weight Loss to Decrease the Risk for Type 2 Diabetes Using Multidimensional Data in Filipino Americans: Secondary Analysis.JMIR diabetes · 2023Article
- Obesity induces morphological and functional changes in female reproductive system through increases in NF-κB and MAPK signaling in mice.Reproductive biology and endocrinology : RB&E · 2021Article
- The Mare: A Pertinent Model for Human Assisted Reproductive Technologies?Animals : an open access journal from MDPI · 2021Review
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
backgroundIncreasing evidence supports a relationship between obesity and either infertility or subfertility in women. Most previous omics studies were focused on determining if the serum and follicular fluid expression profiles of subjects afflicted with both obesity-related infertility and polycystic ovary syndrome (PCOS) are different than those in normal healthy controls. As granulosa cells (GCs) are essential for oocyte development and fertility, we determined here if the protein expression profiles in the GCs from obese subjects are different than those in their normal-weight counterpart.
methodsGC samples were collected from obese female subjects (n = 14) and normal-weight female subjects (n = 12) who were infertile and underwent in vitro fertilization (IVF) treatment due to tubal pathology. A quantitative approach including tandem mass tag labeling and liquid chromatography tandem mass spectrometry (TMT) was employed to identify differentially expressed proteins. Gene Ontology (GO) and the Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses were then conducted to interrogate the functions and pathways of identified proteins. Clinical, hormonal, and biochemical parameters were also analyzed in both groups.
resultsA total of 228 differentially expressed proteins were noted, including 138 that were upregulated whereas 90 others were downregulated. Significant pathways and GO terms associated with protein expression changes were also identified, especially within the mitochondrial electron transport chain. The levels of free fatty acids in both the serum and follicular fluid of obese subjects were significantly higher than those in matched normal-weight subjects.
conclusionsIn GCs obtained from obese subjects, their mitochondria were damaged and the endoplasmic reticulum stress response was accompanied by dysregulated hormonal synthesis whereas none of these changes occurred in normal-weight subjects. These alterations may be related to the high FFA and TG levels detected in human follicular fluid.
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