ArticleBMC genomics2021
Prevalence, antibiotic resistance, virulence and genetic diversity of Staphylococcus aureus isolated from bulk tank milk samples of U.S. dairy herds.
Article in BMC genomics, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers, 1 of them a synthesis that pooled it.
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35 citing papers in PubMed, 1 synthesis or guideline pooled it, 65 citations in OpenAlex.
- The prevalence of bovine mastitis-associatedFrontiers in veterinary science · 2022Pooled it
- MRSA-dominant colonization and synergistic pathogenicity withJournal of orthopaedic translation · 2026Article
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- Genetic diversity, virulence factors and antimicrobial resistance profiles of S. aureus isolated from raw meat, meat products and veterinary samples of food-producing animals in South African provinces.BMC microbiology · 2026Article
- Whole-genome analysis and phenotypic antimicrobial resistance ofFrontiers in microbiology · 2026Article
- Insights into the dynamic cell associated and secreted proteome of Staphylococcus aureus cultured in TSB and milk media: a proteomic analysis.BMC microbiology · 2025Article
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- Antimicrobial resistance profiles and detection of mecA, blaCTX-M-1, and blaSHV genes in bacteria among diabetic foot ulcer patients from selected referral hospitals in Uganda.BMC infectious diseases · 2025Article
- Antimicrobial resistance and molecular characteristics of bovine mastitis-associated methicillin-resistantMicrobiology spectrum · 2025Article
- Prevalence and Characterization ofMicroorganisms · 2025Article
- Whole genome sequencing identifies exotoxin and antimicrobial resistance profiles of Staphylococcus aureus from Maine dairy farms.BMC veterinary research · 2025Article
- Antibiotic susceptibility and molecular characterization based on whole-genome sequencing of Staphylococcus aureus causing invasive infection in children and women living in Southwest China during 2018-2023.BMC microbiology · 2025Article
- Occurrence and diagnostic of intermittent shedding ofFrontiers in veterinary science · 2025Review
- Virulence factors of bovine mastitis pathogens: distribution, pathogenesis, and emerging vaccines targeting virulence factors: a literature review.Frontiers in veterinary science · 2025Review
- Exploring the phylogenetic diversity and antimicrobial activity of non-Frontiers in microbiology · 2025Article
- Investigation of Various Toxigenic Genes and Antibiotic and Disinfectant Resistance Profiles ofFoods (Basel, Switzerland) · 2024Article
- Microbiological and Molecular Investigation of Antimicrobial Resistance inAnimals : an open access journal from MDPI · 2024Article
- Methicillin-ResistantAntibiotics (Basel, Switzerland) · 2024Review
- The "best practices for farming" successfully contributed to decrease the antibiotic resistance gene abundances within dairy farms.Frontiers in veterinary science · 2024Article
- Article
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundColonization of dairy cows by Staphylococcus aureus (S. aureus), especially those which are multi-drug resistant and toxin producing, is a concern for animal health and well-being as well as public health. The objective of this study was to investigate the prevalence, antibiotic resistance, gene content and virulence determinants of S. aureus in bulk tank milk samples (BTM) from U.S. dairy herds.
resultsBTM samples were collected, once in winter and once in summer, from 189 U.S. dairy herds. Of 365 BTM samples cultured, the sample and herd prevalence of S. aureus in BTM was 46.6% (170 of 365 samples) and 62.4% (118 of 189 herds), respectively. Among a subset of 138 S. aureus isolates that were stored for further analysis, 124 were genome sequenced after being confirmed as S. aureus using phenotypic tests. The most commonly identified antimicrobial resistance-associated gene was norA (99.2%) and mecA gene responsible for methicillin resistance (MRSA) was identified in one isolate (0.8%). The most frequently detected putative virulence genes were aur (100%), hlgB (100%), hlgA, hlgC, hlb (99.2%), lukE (95.9%) and lukD (94.3%). In the 53 staphylococcal enterotoxin positive isolates, sen (37.9%), sem (35.5%), sei (35.5%) and seg (33.1%) were the most frequently detected enterotoxin genes. Among the 14 sequence types (ST) and 18 spa types identified, the most common was ST2187 (20.9%) and t529 (28.2%), respectively. The most predominant clone was CC97 (47.6%) followed by CC unknown (36.3%). The single MRSA isolate belonged to ST72-CC8, spa type t126 and was negative for the tst gene but harbored all the other virulence genes investigated.
conclusionOur findings indicated a high prevalence of S. aureus in BTM of U.S. dairy herds, with isolates showing little evidence of resistance to antibiotics commonly used to treat mastitis. However, isolates often carried genes for the various enterotoxins. This study identified predominant genetic clones. Despite lower prevalence, the presence of MRSA and multi-drug resistant strains in BTM poses a significant risk to animal and public health if their number were to increase in dairy environment. Therefore, it is necessary to continuously monitor the use of antibiotics in dairy cows.
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