ReviewThe Journal of biological chemistry2021
Seeing beyond the limit: A guide to choosing the right super-resolution microscopy technique.
Review in The Journal of biological chemistry, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 76 papers.
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Who cites it
76 citing papers in PubMed.
- A practical workflow for analyzing organelle spatial proximity with super-resolution microscopy.Biochemistry and biophysics reports · 2026Article
- Article
- Breaking the 100-nm resolution barrier with multiphoton microscopy using image scanning microscopy and optical fluctuation imaging.Journal of biomedical optics · 2026Article
- Photonic waveguide chip-based nanoscopy visualizes rearrangements of the cortical actin cytoskeleton in activated Jurkat T cells.Science advances · 2026Article
- Nanoscale Mapping Reveals Periodic Organization of Neutrophil Extracellular Trap Proteins.Nano letters · 2026Article
- From Microscopy to Nanoscopy: Contemporary Physical Methods in Mitochondrial Structural Biology.International journal of molecular sciences · 2026Review
- Establishing functional giant Dictyostelium cells reveals front-rear polarity in intracellular signaling.Communications biology · 2026Article
- Self-Driving Microscopes: AI Meets Super-Resolution Microscopy.Small methods · 2026Review
- Case Study: Illuminating the Nanoscale World of Microbiology.Methods in molecular biology (Clifton, N.J.) · 2026Review
- Localization of Proteins in the Secretory Pathway of Pichia pastoris Using Fluorescence Microscopy.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Exploring Biophysics at the Membrane with Single-Molecule TIRF Microscopy.Advances in experimental medicine and biology · 2026Review
- BlinkFusion: modular platform quantifying labeling efficiency and photophysics in regular and super-resolution fluorescence microscopy.Frontiers in bioinformatics · 2026Article
- Expansion and fluctuations-enhanced microscopy for nanoscale molecular profiling of cells and tissues.Nature protocols · 2026Review
- Nanoscale Imaging of Biological Tissues: Techniques, Challenges and Emerging Frontiers.Nanomaterials (Basel, Switzerland) · 2025Review
- Transition metal complexes as optical probes for super-resolution microscopy.Nature reviews. Chemistry · 2025Review
- Nanobiosensors for Single-Molecule Diagnostics: Toward Integration with Super-Resolution Imaging.Biosensors · 2025Review
- Advanced optical microscopy methods forBiophysical reviews · 2025Review
- Advancements in Single-Molecule Fluorescence Detection Techniques and Their Expansive Applications in Drug Discovery and Neuroscience.Biosensors · 2025Review
- A Bayesian Model to Count the Number of Two-State Emitters in a Diffraction Limited Spot.Nano letters · 2025Article
- Tsang's resolution enhancement method for imaging with focused illumination.Light, science & applications · 2025Article
16 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Super-resolution microscopy has become an increasingly popular and robust tool across the life sciences to study minute cellular structures and processes. However, with the increasing number of available super-resolution techniques has come an increased complexity and burden of choice in planning imaging experiments. Choosing the right super-resolution technique to answer a given biological question is vital for understanding and interpreting biological relevance. This is an often-neglected and complex task that should take into account well-defined criteria (e.g., sample type, structure size, imaging requirements). Trade-offs in different imaging capabilities are inevitable; thus, many researchers still find it challenging to select the most suitable technique that will best answer their biological question. This review aims to provide an overview and clarify the concepts underlying the most commonly available super-resolution techniques as well as guide researchers through all aspects that should be considered before opting for a given technique.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.