ArticleJournal of the American Chemical Society2021
Hydrophobic Interactions between DNA Duplexes and Synthetic and Biological Membranes.
Article in Journal of the American Chemical Society, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed.
- A programmable DNA origami nanosyringe for directed membrane translocation.Nature nanotechnology · 2026Article
- Membrane-Associated Biomolecules for Synthetic Cell Signalling.Chembiochem : a European journal of chemical biology · 2026Review
- Data-Driven Electrochemistry Reveals the Impact of Hydrophobicity on Aptamer Cross-Reactivity.ACS measurement science au · 2026Article
- Membrane-Penetrating Molecular Device Based on a Triplex Containing Acyclic L-Threoninol Nucleic Acid Functionalized with Cholesterol.Angewandte Chemie (International ed. in English) · 2026Article
- pH-responsive synthetic cells for controlled protein synthesis and release.bioRxiv : the preprint server for biology · 2025Article
- Small amphiphilic DNA for programmable transmembrane signaling and amplification.bioRxiv : the preprint server for biology · 2025Article
- On-the-Fly Microfluidic Control of Giant Vesicle Compositions Validated by DNA Surface Charge Sensors.ACS nano · 2025Article
- Modulating the DNA/Lipid Interface through Multivalent Hydrophobicity.Nano letters · 2024Article
- Solid Phase Oligo-DNA Extraction from Complex Medium Using an Aminated Graphene/Nitrocellulose Membrane Hybrid.Biomolecules · 2024Article
- Nanoparticle-mediated dsRNA delivery for precision insect pest control: a comprehensive review.Molecular biology reports · 2024Review
- Advancing cell surface modification in mammalian cells with synthetic molecules.Chemical science · 2023Review
- Dynamic Fusion of Nucleic Acid Functionalized Nano-/Micro-Cell-Like Containments: From Basic Concepts to Applications.ACS nano · 2023Review
- Lipid-linked nucleoside triphosphates for enzymatic synthesis of hydrophobic oligonucleotides with enhanced membrane anchoring efficiency.Chemical science · 2023Article
- Modulating membrane fusion through the design of fusogenic DNA circuits and bilayer composition.Soft matter · 2022Article
- Conjugation of chemical handles and functional moieties to DNA during solid phase synthesis with sulfonyl azides.Nucleic acids research · 2022Article
- The interactions between DNA nanostructures and cells: A critical overview from a cell biology perspective.Acta biomaterialia · 2022Review
- Digging into the biophysical features of cell membranes with lipid-DNA conjugates.Quarterly reviews of biophysics · 2022Review
- Site-specific DNA functionalization through the tetrazene-forming reaction in ionic liquids.Chemical science · 2022Article
- A Surfactant Enables Efficient Membrane Spanning by Non-Aggregating DNA-Based Ion Channels.Molecules (Basel, Switzerland) · 2022Article
- Binding of DNA origami to lipids: maximizing yield and switching via strand displacement.Nucleic acids research · 2021Article
Corrections and comments
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Authors and funding
7 authors.
Funding
Abstract
Equipping DNA with hydrophobic anchors enables targeted interaction with lipid bilayers for applications in biophysics, cell biology, and synthetic biology. Understanding DNA-membrane interactions is crucial for rationally designing functional DNA. Here we study the interactions of hydrophobically tagged DNA with synthetic and cell membranes using a combination of experiments and atomistic molecular dynamics (MD) simulations. The DNA duplexes are rendered hydrophobic by conjugation to a terminal cholesterol anchor or by chemical synthesis of a charge-neutralized alkyl-phosphorothioate (PPT) belt. Cholesterol-DNA tethers to lipid vesicles of different lipid compositions and charges, while PPT DNA binding strongly depends on alkyl length, belt position, and headgroup charge. Divalent cations in the buffer can also influence binding. Our MD simulations directly reveal the complex structure and energetics of PPT DNA within a lipid membrane, demonstrating that longer alkyl-PPT chains provide the most stable membrane anchoring but may disrupt DNA base paring in solution. When tested on cells, cholesterol-DNA is homogeneously distributed on the cell surface, while alkyl-PPT DNA accumulates in clustered structures on the plasma membrane. DNA tethered to the outside of the cell membrane is distinguished from DNA spanning the membrane by nuclease and sphingomyelinase digestion assays. The gained fundamental insight on DNA-bilayer interactions will guide the rational design of membrane-targeting nanostructures.
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