ArticleAngewandte Chemie (International ed. in English)2021
Particle-by-Particle In Situ Characterization of the Protein Corona via Real-Time 3D Single-Particle-Tracking Spectroscopy*.
Article in Angewandte Chemie (International ed. in English), 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 28 citations in OpenAlex.
- Fractionation-Free Protein Corona Quantification Through Synchrotron-Based Small-Angle X-ray Scattering.Small methods · 2026Article
- 3D Single-Virus Tracking: Advances in Methodology and Labeling Strategies Towards Probing the Virus-Epithelium Interaction.Viruses · 2026Review
- Evaluating the In Situ Effects of Whole Protein Coronas on the Biosensing of Antibody-Immobilized Nanoparticles Using Two-Color Fluorescence Nanoparticle Tracking Analysis.Nanomaterials (Basel, Switzerland) · 2025Article
- Review
- Protein Corona of Nanoparticles: Isolation and Analysis.Chem & bio engineering · 2024Review
- Single-nanoparticle electrophoretic mobility determination and trapping using active-feedback 3D tracking.bioRxiv : the preprint server for biology · 2024Article
- Article
- Hour-Long, Kilohertz Sampling Rate Three-Dimensional Single-Virus Tracking in Live Cells Enabled by StayGold Fluorescent Protein Fusions.The journal of physical chemistry. B · 2024Article
- Hour-long, Kilohertz Sampling Rate 3D Single-virus Tracking in Live Cells Enabled by StayGold Fluorescent Protein Fusions.bioRxiv : the preprint server for biology · 2024Article
- Probing the Cellular Fate of the Protein Corona around Nanoparticles with Nanofocused X-ray Fluorescence Imaging.International journal of molecular sciences · 2023Article
- Real-Time Optical Tracking of Protein Corona Formation on Single Nanoparticles in Serum.ACS nano · 2023Article
- Active-Feedback 3D Single-Molecule Tracking Using a Fast-Responding Galvo Scanning Mirror.The journal of physical chemistry. A · 2023Article
- Simultaneous Sizing and Refractive Index Analysis of Heterogeneous Nanoparticle Suspensions.ACS nano · 2023Article
- Combined online Bayesian and windowed estimation of background and signal localization facilitates active-feedback particle tracking in complex environments.The Journal of chemical physics · 2022Article
- In situ analysis of nanoparticle soft corona and dynamic evolution.Nature communications · 2022Article
- Real-Time Feedback-Driven Single-Particle Tracking: A Survey and Perspective.Small (Weinheim an der Bergstrasse, Germany) · 2022Review
- Dynamic intracellular exchange of nanomaterials' protein corona perturbs proteostasis and remodels cell metabolism.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Nanoparticles (NPs) adsorb proteins when exposed to biological fluids, forming a dynamic protein corona that affects their fate in biological environments. A comprehensive understanding of the protein corona is lacking due to the inability of current techniques to precisely measure the full corona in situ at the single-particle level. Herein, we introduce a 3D real-time single-particle tracking spectroscopy to "lock-on" to single freely diffusing polystyrene NPs and probe their individual protein coronas, primarily using bovine serum albumin (BSA) as a model system. The fluorescence signals and diffusive motions of the tracked NPs enable quantification of the "hard corona" using mean-squared displacement analysis. Critically, this method's particle-by-particle nature enabled a lock-in-type frequency filtering approach to extract the full protein corona, despite the typically confounding effect of high background signal from unbound proteins. From these results, the dynamic in situ full protein corona is observed to contain twice the number of proteins compared to the ex situ-measured "hard" protein corona.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.