ArticleClinical chemistry and laboratory medicine2021
Simultaneous measurement of 13 circulating vitamin D3 and D2 mono and dihydroxy metabolites using liquid chromatography mass spectrometry.
Article in Clinical chemistry and laboratory medicine, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed.
- Exercise without Weight Loss Prevents Seasonal Decline in Vitamin D Metabolites: The VitaDEx Randomized Controlled Trial.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Trial
- Interlaboratory comparison with a reference measurement procedure (RMP) for determining 24,25-dihydroxyvitamin DAnalytical and bioanalytical chemistry · 2026Article
- The vitamin DAmerican journal of physiology. Cell physiology · 2025Article
- Vitamin - D deficiency as a risk factor in primary hypertension - A cross sectional study.Bioinformation · 2025Article
- Anticancer Activity of Vitamin D, Lumisterol and Selected Derivatives against Human Malignant Melanoma Cell Lines.International journal of molecular sciences · 2024Article
- Biological Effects of CYP11A1-Derived Vitamin D and Lumisterol Metabolites in the Skin.The Journal of investigative dermatology · 2024Review
- Review
- Photo-neuro-immuno-endocrinology: How the ultraviolet radiation regulates the body, brain, and immune system.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Article
- Recent Advances in Vitamin D Biology: Something New under the Sun.The Journal of investigative dermatology · 2023Article
- Determination of 24,25-dihydroxyvitamin DThe Journal of steroid biochemistry and molecular biology · 2023Article
- Serum Vitamin D Metabolites by HPLC-MS/MS Combined with Differential Ion Mobility Spectrometry: Aspects of Sample Preparation without Derivatization.International journal of molecular sciences · 2023Article
- Vitamin D Metabolites: Analytical Challenges and Clinical Relevance.Calcified tissue international · 2023Review
- Novel CYP11A1-Derived Vitamin D and Lumisterol Biometabolites for the Management of COVID-19.Nutrients · 2022Review
- Metabolic activation of tachysterolFASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2022Article
- CYP11A1‑derived vitamin D hydroxyderivatives as candidates for therapy of basal and squamous cell carcinomas.International journal of oncology · 2022Article
- Consideration of possible effects of vitamin D on established cancer, with reference to malignant melanoma.Pigment cell & melanoma research · 2022Review
- Chemical synthesis, biological activities and action on nuclear receptors of 20S(OH)DBioorganic chemistry · 2022Article
- Circulating Conjugated and Unconjugated Vitamin D Metabolite Measurements by Liquid Chromatography Mass Spectrometry.The Journal of clinical endocrinology and metabolism · 2022Article
- Low Vitamin D Status Predicts Poor Clinical Outcome in Advanced Melanoma Treated With Immune Checkpoint or BRAF/MEK Inhibitors: A Prospective Non-Interventional Side-by-Side Analysis.Frontiers in oncology · 2022Article
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
objectivesClinical evaluation of vitamin D status is conventionally performed by measuring serum levels of a single vitamin D metabolite, 25-hydroxyvitamin D predominantly by immunoassay methodology. However, this neglects the complex metabolic pathways involved in vitamin D bioactivity, including two canonical forms D3 and D2, bioactive 1,25-dihydroxy metabolites and inactive 24-hydroxy and other metabolites.
methodsLiquid chromatography-tandem mass spectrometry (LC-MS/MS) can measure multiple analytes in a sample during a single run with high sensitivity and reference level specificity. We therefore aimed to develop and validate a LC-MS/MS method to measure simultaneously 13 circulating vitamin D metabolites and apply it to 103 human serum samples.
resultsThe LC-MS/MS method using a Cookson-type derivatization reagent phenyl-1,2,4-triazoline-3,5-dione (PTAD) quantifies 13 vitamin D metabolites, including mono and dihydroxy-metabolites, as well as CYP11A1-derived D3 and D2 metabolites in a single run. The lower limit of quantitation was 12.5 pg/mL for 1,25(OH)
conclusionsThis profiling method can provide new insight into circulating vitamin D metabolite pathways forming the basis for improved understanding of the role of vitamin D in health and disease.
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