ArticleAvicenna journal of medical biotechnology
Designing Two Synthetic Constructs for Real Time PCR Detection of
Article in Avicenna journal of medical biotechnology. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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4 authors at 3 institutions in 1 country.
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Abstract
backgroundGenerally, timely diagnosis of micro-organisms is very important to prevent many diseases. Many methods can detect micro-organisms like culture-based methods and molecular methods. The molecular methods are usually preferred because they provide fast and reliable results. In some cases, microbial strains are not accessible, and there is no safety to work with them; therefore, synthetic constructs which are designed according to the available sequences in databases can be used as a positive control for detection of them.
methodsIn this study, a synthetic construct was designed for molecular detection of
resultsThe synthetic construct was designed and used for conventional PCR and multiplex PCR. The results of common PCR showed a single band at 148
conclusionIn conclusion, the designed construct can be used as a positive control for an accurate diagnosis of these micro-organisms without any biological danger for laboratory staff. So, this method is useful for diagnosis of these agents in food, water, and blood samples.
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