Evidence map›Paper›PMID 34011267›Full record

ArticleBasic and clinical andrology2021

LRWD1 expression is regulated through DNA methylation in human testicular embryonal carcinoma cells.

Jui-Hsiang Hung, Han-Yi Cheng, Yung-Chieh Tsai, Hsien-An Pan, Hany A Omar, Chien-Chih Chiu, Yin-Mei Su, Yung-Ming Lin, Yen-Ni Teng

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Article in Basic and clinical andrology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

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4 · The record

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5 · Who and what money

Authors and funding

9 authors.

Jui-Hsiang HungDepartment of Biotechnology, Chia Nan University of Pharmacy and Science, Tainan, Taiwan.
Han-Yi ChengDepartment of Biological Sciences and Technology, National University of Tainan, No.33, Sec. 2, Shulin St., West Central District, Tainan City, 700, Taiwan.
Yung-Chieh TsaiDepartment of Obstetrics and Gynecology, Chi-Mei Medical Center; Department of Sport Management, and Department of Biotechnology, Chia Nan University of Pharmacy and Science, Tainan, Taiwan.
Hsien-An PanAn-An Women and Children clinic, Tainan, Taiwan.
Hany A OmarSharjah Institute for Medical Research and College of Pharmacy, University of Sharjah, Sharjah, 27272, United Arab Emirates.
Chien-Chih ChiuDepartment of Biotechnology, Kaohsiung Medical University, Kaohsiung, Taiwan.
Yin-Mei SuDepartment of Biological Sciences and Technology, National University of Tainan, No.33, Sec. 2, Shulin St., West Central District, Tainan City, 700, Taiwan.
Yung-Ming LinDepartment of Obstetrics and Gynecology, Faculty of Medicine, University of British Columbia, Vancouver, British Columbia, Canada.
Yen-Ni TengDepartment of Biological Sciences and Technology, National University of Tainan, No.33, Sec. 2, Shulin St., West Central District, Tainan City, 700, Taiwan. tengyenni@mail.nutn.edu.tw.ORCID http://orcid.org/0000-0002-4670-8149

Funding

Ministry of Science and Technology, Taiwan 104-2314-B-024-002-MY2Ministry of Science and Technology, Taiwan 106-2314-B-024-001Ministry of Science and Technology, Taiwan 107-2314-B-024-001Ministry of Science and Technology, Taiwan 108-2314-B-024-001
6 · The paper itself

Abstract

backgroundSperm growth and maturation are correlated with the expression levels of Leucine-rich repeat and WD repeat-containing protein 1 (LRWD1), a widely expressed protein in the human testicles. The decrease in LRWD1 cellular level was linked to the reduction in cell growth and mitosis and the rise in cell microtubule atrophy rates. Since DNA methylation has a major regulatory role in gene expression, this study aimed at exploring the effect of the modulation of DNA methylation on LRWD1 expression levels.

resultsThe results revealed the presence of a CpG island up of 298 bps (- 253 ~ + 45) upon LRWD1 promoter in NT2/D1 cells. The hypermethylation of the LRWD1 promoter was linked to a reduction in the transcription activity in NT2/D1 cells, as indicated by luciferase reporter assay. The methylation activator, floxuridine, confirmed the decrease in the LRWD1 promoter transcriptional activity. On the other hand, 5-Aza-2'-deoxycytidine (5-Aza-dc, methylation inhibitor), significantly augmented LRWD1 promoter activity and the expression levels of mRNA and proteins. Furthermore, DNA methylation status of LRWD1 promoter in human sperm genomic DNA samples was analyzed. The results indicated that methylation of LRWD1 promoter was correlated to sperm activity.

conclusionsThus, the regulation of LRWD1 expression is correlated with the methylation status of LRWD1 promoter, which played a significant role in the modulation of spermatogenesis, sperm motility, and vitality. Based on these results, the methylation status of LRWD1 promoter may serve as a novel molecular diagnostic marker or a therapeutic target in males' infertility.

Indexed as

5-Aza-2′-deoxycytidineDNA methylationFloxuridineLRWD1

Identifiers

PMID34011267
PMCPMC8136200

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