Evidence map›Paper›PMID 34010687›Full record

ArticleThe Journal of steroid biochemistry and molecular biology2021

Vitamin D Standardization Program (VDSP) intralaboratory study for the assessment of 25-hydroxyvitamin D assay variability and bias.

Stephen A Wise, Johanna E Camara, Christopher T Sempos, Pierre Lukas, Caroline Le Goff, Stephanie Peeters, Carolyn Q Burdette, Federica Nalin, Grace Hahm, Ramón A Durazo-Arvizu and 3 more

Open access · greenAbstract readEvaluation Study
PubMed Full text at PMC Publisher
In one paragraph

Article in The Journal of steroid biochemistry and molecular biology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers, 4 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
35citing papers in PubMed, 4 pooled it
5.2field-weighted citation impact, top 4% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

35 citing papers in PubMed, 4 syntheses or guidelines pooled it, 47 citations in OpenAlex.

  1. Pooled it
  2. The Lebanese GRADE-based vitamin D guidelines: a paradigm for the MENA region.Osteoporosis international : a journal established as result of cooperation between the European Foundation for Osteoporosis and the National Osteoporosis Foundation of the USA · 2025
    Guideline
  3. Pooled it
  4. Shift Work and Serum Vitamin D Levels: A Systematic Review and Meta-Analysis.International journal of environmental research and public health · 2022
    Pooled it
  5. Association of standardized serum 25-hydroxyvitamin D with falls in post-menopausal women.Osteoporosis international : a journal established as result of cooperation between the European Foundation for Osteoporosis and the National Osteoporosis Foundation of the USA · 2025
    Trial
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors at 4 institutions in 2 countries.

Stephen A WiseOffice of Dietary Supplements (ODS), National Institutes of Health (NIH), Bethesda, MD 20892, USA; Scientist Emeritus, Associate, National Institute of Standards and Technology (NIST), Gaithersburg, MD 20899, USA. Electronic address: stephen.wise@nih.gov.
Johanna E CamaraChemical Sciences Division, National Institute of Standards and Technology (NIST), Gaithersburg, MD 20899, USA.
Christopher T SemposOffice of Dietary Supplements (ODS), National Institutes of Health (NIH), Bethesda, MD 20892, USA; Vitamin D Standardization Program LLC, Havre de Grace, MD 21078 USA.
Pierre LukasClinical Chemistry, University of Liège, CHU de Liège, B-4000 Liège, Belgium.
Caroline Le GoffClinical Chemistry, University of Liège, CHU de Liège, B-4000 Liège, Belgium.
Stephanie PeetersClinical Chemistry, University of Liège, CHU de Liège, B-4000 Liège, Belgium.
Carolyn Q BurdetteChemical Sciences Division, National Institute of Standards and Technology (NIST), Gaithersburg, MD 20899, USA.
Federica NalinChemical Sciences Division, National Institute of Standards and Technology (NIST), Gaithersburg, MD 20899, USA.
Grace HahmChemical Sciences Division, National Institute of Standards and Technology (NIST), Gaithersburg, MD 20899, USA.
Ramón A Durazo-ArvizuBiostatistics Core, The Sabin Research Institute, Keck School of Medicine, University of Southern California, Los Angeles, CA 90027, USA.
Adam J KuszakOffice of Dietary Supplements (ODS), National Institutes of Health (NIH), Bethesda, MD 20892, USA.
Joyce MerkelOffice of Dietary Supplements (ODS), National Institutes of Health (NIH), Bethesda, MD 20892, USA.
Étienne CavalierClinical Chemistry, University of Liège, CHU de Liège, B-4000 Liège, Belgium.
National Institute of Standards and Technology · USNational Institutes of Health · USUniversity of Liège · BEUniversity of Southern California · US

Funding

Intramural NIH HHS Z99 OD999999
6 · The paper itself

Abstract

An intralaboratory study assessing assay variability and bias for determination of serum total 25-hydroxyvitamin D [25(OH)D] was conducted by the Vitamin D Standardization Program (VDSP). Thirteen assays for serum total 25(OH)D were evaluated in a single laboratory including 11 unique immunoassays and one liquid chromatography - tandem mass spectrometry (LC-MS/MS) assay. Fifty single-donor serum samples, including eight samples with high concentrations of 25(OH)D

Indexed as

BiasBiological AssayChromatography, LiquidHumansImmunoassayLaboratoriesReference StandardsReproducibility of ResultsTandem Mass SpectrometryVitamin DVitamins25-hydroxyvitamin DVitamin DVitamins25-Hydroxyvitamin D(2)25-Hydroxyvitamin D(3)3-epi-25-hydroxyvitamin D(3)ImmunoassayLiquid chromatography–tandem mass spectrometry (LC–MS/MS)Reference measurement procedure (RMP)Serum total 25-hydroxyvitamin DVitamin D Standardization Program (VDSP)

Identifiers

PMID34010687
PMCPMC8403635
OpenAlexW3161557458

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.