Evidence map›Paper›PMID 34010667›Full record

ArticleJournal of structural biology2021

Integrating on-grid immunogold labeling and cryo-electron tomography to reveal photosystem II structure and spatial distribution in thylakoid membranes.

Jennifer Jiang, Kuan Yu Cheong, Paul G Falkowski, Wei Dai

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of structural biology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
0.4field-weighted citation impact, top 41% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 9 citations in OpenAlex.

  1. Molecular tags for electron cryo-tomography.Emerging topics in life sciences · 2025
    Review
  2. Review
  3. Structural glycobiology - from enzymes to organelles.Biochemical Society transactions · 2025
    Review
  4. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 1 institution in 1 country.

Jennifer JiangDepartment of Cell Biology and Neuroscience, Rutgers, The State University of New Jersey, Piscataway, NJ 08854, United States; Institute for Quantitative Biomedicine, Rutgers, The State University of New Jersey, Piscataway, NJ 08854, United States.
Kuan Yu CheongEnvironmental Biophysics and Molecular Ecology Program, Department of Marine and Coastal Sciences, Rutgers, The State University of New Jersey, New Brunswick, NJ 08901, United States; Department of Plant Biology, Rutgers, The State University of New Jersey, New Brunswick, NJ 08901, United States.
Paul G FalkowskiInstitute for Quantitative Biomedicine, Rutgers, The State University of New Jersey, Piscataway, NJ 08854, United States; Environmental Biophysics and Molecular Ecology Program, Department of Marine and Coastal Sciences, Rutgers, The State University of New Jersey, New Brunswick, NJ 08901, United States; Department of Earth and Planetary Sciences, Rutgers, The State University of New Jersey, Piscataway, NJ 08854, United States.
Wei DaiDepartment of Cell Biology and Neuroscience, Rutgers, The State University of New Jersey, Piscataway, NJ 08854, United States; Institute for Quantitative Biomedicine, Rutgers, The State University of New Jersey, Piscataway, NJ 08854, United States. Electronic address: wei.dai@rutgers.edu.
Rutgers, The State University of New Jersey · US

Funding

Midwest Consortium for High Resolution Cryoelectron MicroscopyU24GM116789 · NIGMS · PURDUE UNIVERSITY · PI BULLITT, ESTHER, DAI, WEI · 2017 to 2021
$3.5M
NIGMS NIH HHS U24 GM116789
6 · The paper itself

Abstract

A long-standing challenge in cell biology is elucidating the structure and spatial distribution of individual membrane-bound proteins, protein complexes and their interactions in their native environment. Here, we describe a workflow that combines on-grid immunogold labeling, followed by cryo-electron tomography (cryoET) imaging and structural analyses to identify and characterize the structure of photosystem II (PSII) complexes. Using an antibody specific to a core subunit of PSII, the D1 protein (uniquely found in the water splitting complex in all oxygenic photoautotrophs), we identified PSII complexes in biophysically active thylakoid membranes isolated from a model marine diatom Phaeodactylum tricornutum. Subsequent cryoET analyses of these protein complexes resolved two PSII structures: supercomplexes and dimeric cores. Our integrative approach establishes the structural signature of multimeric membrane protein complexes in their native environment and provides a pathway to elucidate their high-resolution structures.

Indexed as

DiatomsThylakoidsElectron Microscope TomographyLight-Harvesting Protein ComplexesPhotosystem II Protein ComplexLight-Harvesting Protein ComplexesPhotosystem II Protein ComplexCryo-electron tomography (cryoET)Immunogold labelingPhotosynthesisPhotosystem II (PSII)Protein identificationSpatial distribution

Identifiers

PMID34010667
PMCPMC8577061
OpenAlexW3161544903

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.