Evidence map›Paper›PMID 34009563›Full record

ArticleMolecular biology reports2021

(3,3'-Methylene)bis-2-hydroxy-1,4-naphthoquinones induce cytotoxicity against DU145 and PC3 cancer cells by inhibiting cell viability and promoting cell cycle arrest.

Paula Priscilla de Freitas, Ruan Carlos Busquet Ribeiro, Isabella Dos Santos Guimarães, Caroline S Moreira, David R Rocha, Fernando de Carvalho da Silva, Vitor Francisco Ferreira, Etel Rodrigues Pereira Gimba

Abstract read
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In one paragraph

Article in Molecular biology reports, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.6field-weighted citation impact, top 39% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 4 citations in OpenAlex.

  1. Review
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 2 institutions in 1 country.

Paula Priscilla de FreitasInstituto Nacional de Câncer, Coordenação de Pesquisa, Centro, Rio de Janeiro, RJ, 20231-050, Brazil.
Ruan Carlos Busquet RibeiroInstituto de Química, Universidade Federal Fluminense, Campus do Valonguinho, Niterói, RJ, 24020-150, Brazil.
Isabella Dos Santos GuimarãesDivisão de Pesquisa Clínica e Desenvolvimento Tecnológico, Laboratório de Pesquisa Translacional, Instituto Nacional de Câncer, Rio de Janeiro, RJ, Brazil.
Caroline S MoreiraInstituto de Química, Universidade Federal Fluminense, Campus do Valonguinho, Niterói, RJ, 24020-150, Brazil.
David R RochaInstituto de Química, Universidade Federal Fluminense, Campus do Valonguinho, Niterói, RJ, 24020-150, Brazil.
Fernando de Carvalho da SilvaInstituto de Química, Universidade Federal Fluminense, Campus do Valonguinho, Niterói, RJ, 24020-150, Brazil.
Vitor Francisco FerreiraDepartamento de Tecnologia Farmacêutica, Faculdade de Farmácia, Universidade Federal Fluminense, Santa Rosa, Niterói, RJ, 24241-002, Brazil.
Etel Rodrigues Pereira GimbaInstituto Nacional de Câncer, Coordenação de Pesquisa, Centro, Rio de Janeiro, RJ, 20231-050, Brazil. etelgimba@id.uff.br.ORCID http://orcid.org/0000-0001-7091-2206
Universidade Federal Fluminense · BRInstituto Nacional de Câncer - INCA · BR

Funding

CNPQ 310591/2014-7 and 312158/2017-3FAPERJ E-26/201.448/2014, E-26/E-26/111.227/2014; 210.394/2014; E-26/010.002007/2014; E-26/203.204/2015; E-26/203.006/2018FCT/CAPES 99999.008550/2014-00Programa de Oncobiologia/UFRJ not apllicable
6 · The paper itself

Abstract

We developed a novel method for the synthesis of bis-naphthoquinones (BNQ), which are hybrids of lawsone (2-hydroxy-1,4-naphthoquinone) and 3-hydroxy-juglone (3,5-dihydroxy-1,4-naphthoquinone). The anticancer activity of three synthesized compounds, named 4 (RC10), 5 (RCDFC), and 6 (RCDOH) was evaluated in vitro against two metastatic prostate cancer (PCa) cell lines, DU145 and PC3, using MTT assays. We found that 4 (RC10) and 5 (RCDFC) induced cytotoxicity against DU145 and PC3 cells. Flow cytometry analysis revealed that these two compounds promoted cell cycle arrest in G1/S and G2/M phases, increased Sub-G1 peak and induced inhibition in cell viability. We also showed that these effects are cell-type context dependent and more selective for these tested PCa cells than for HUVEC non-tumor cells. The two BNQ compounds 4 (RC10) and 5 (RCDFC) displayed promising anticancer activity against the two tested metastatic PCa cell lines, DU145 and PC3. Their effects are mainly associated with inhibition of cell viability, possibly through apoptotic cell death, besides altering the SubG1, G1/S and G2/M phases of cell cycle. 5 (RCDFC) compound was found to be more selective than 4 (RC10), when comparing their cytotoxic effects in relation to HUVEC non-tumoral cells. Future work should also test these compounds in combination with other chemotherapeutic drugs to evaluate their effects on further sensitizing drug-resistant metastatic PCa cells.

Indexed as

Antineoplastic AgentsNaphthoquinonesApoptosisCell CycleCell Cycle CheckpointsCell Line, TumorCell ProliferationCell SurvivalDrug Screening Assays, AntitumorHumansMalePC-3 CellsProstatic NeoplasmsAntineoplastic AgentsjuglonelawsoneNaphthoquinonesCancer cellJugloneKnoevenagel reactionLawsoneMannich reactionPca cell linesQuinone methides

Identifiers

PMID34009563
OpenAlexW3161636370

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.