Evidence map›Paper›PMID 34007282›Full record

ArticleInternational journal of microbiology2021

Production, Optimization, and Characterization of an Acid Protease from a Filamentous Fungus by Solid-State Fermentation.

Abdilbar Usman, Said Mohammed, Jermen Mamo

Open access · goldAbstract read
In one paragraph

Article in International journal of microbiology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.

0numbers the graph read from it
0cells of the map it votes in
10citing papers in PubMed
3.3field-weighted citation impact, top 8% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

10 citing papers in PubMed, 30 citations in OpenAlex.

  1. Article
  2. Article
  3. A Novel Chymotrypsin-like Protease fromFoods (Basel, Switzerland) · 2026
    Article
  4. Article
  5. Review
  6. Article
  7. Article
  8. Upgrading the Bioactive Potential of Hazelnut Oil Cake byMolecules (Basel, Switzerland) · 2024
    Article
  9. Article
  10. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 1 institution in 1 country.

Abdilbar UsmanDepartment of Biology, College of Natural and Computational Science, Debre Berhan University, Debre Berhan, Ethiopia.
Said MohammedDepartment of Biology, College of Natural and Computational Science, Debre Berhan University, Debre Berhan, Ethiopia.
Jermen MamoDepartment of Biology, College of Natural and Computational Science, Debre Berhan University, Debre Berhan, Ethiopia.ORCID https://orcid.org/0000-0003-0776-3310
Debre Berhan University · ET

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Acid proteases represent an important group of enzymes, extensively used in food and beverage industries. There is an increased demand for acid proteases adapting to the industrial extreme environment, especially lower pH. Thus, this necessitates the search for a better acid protease from fungi that best performs in industrial conditions. The fungal isolates were isolated from grape and dairy farm soil using potato dextrose agar and further screened for protease production based on the hydrolysis of clear zone on skim milk agar. The potential fungi were then subjected to secondary screening under solid-state fermentation (SSF). After the secondary screening, the potential fungus was identified to the genus level by the macroscopic and microscopic methods. The growth conditions and media composition for the potential fungus were further optimized under SSF. The crude enzyme produced by the potential isolate was characterized after partial purification by acetone and ammonium sulfate precipitation. A total of 9 fungal isolates showed protease production in primary and secondary screening; however, one potential isolate (Z1BL1) was selected for further study based on its protease activity. The isolate was identified to the genus

Identifiers

PMID34007282
PMCPMC8102121
OpenAlexW3157292235

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.