ArticleScientific reports2021
Development of marker-free transgenic pigeon pea (Cajanus cajan) expressing a pod borer insecticidal protein.
Article in Scientific reports, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
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Who cites it
6 citing papers in PubMed, 11 citations in OpenAlex.
- Bt agave: why it is time to explore a new biotechnological frontier.Pest management science · 2026Review
- Development of a High-Efficiency Hairy Root Transformation System for Diverse Cowpea (Plants (Basel, Switzerland) · 2026Article
- A DEAD box helicase Psp68 positively regulates salt stress responses in marker-free transgenic rice plants.Transgenic research · 2023Article
- Marker-Free Rice (Antioxidants (Basel, Switzerland) · 2022Article
- Clean gene technology to develop selectable marker-free pod borer-resistant transgenic pigeon pea events involving the constitutive expression of Cry1Ac.Applied microbiology and biotechnology · 2022Article
- Development of marker-free insect resistant transgenic okra (Physiology and molecular biology of plants : an international journal of functional plant biology · 2021Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Pigeon pea, a grain legume of the semiarid tropics, is a rich source of high-quality protein. The productivity of this pulse is seriously affected by lepidopteron insect pests. To generate a sustainable insect-resistant plant, synthetically prepared bioactive key constituents of a crystal protein (Syn Cry1Ab) of Bacillus thuringiensis were expressed in pigeon pea under the guidance of a tissue-specific promoter of the RuBP carboxylase/oxygenase small subunit (rbcS) gene. Regenerated transgenic plants with the cry1Ab expression cassette (cry1Ab-lox-bar-lox) showed the optimum insect motility rate (90%) in an in vitro insect bioassay with second instar larvae, signifying the insecticidal potency of Syn Cry1Ab. In parallel, another plant line was also generated with a chimaeric vector harbouring a cre recombinase gene under the control of the CaMV 2 × 35S promoter. Crossing between T
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