ArticleNature communications2021
Guiding functions of the C-terminal domain of topoisomerase IIα advance mitotic chromosome assembly.
Article in Nature communications, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 40 citations in OpenAlex.
- Dual phosphoregulatory mechanisms of condensin I revealed by biochemical reconstitution.PNAS nexus · 2026Article
- Histone Density and Dynamics Shape Mitotic Chromatid Architecture in Xenopus Egg Extracts.Genes to cells : devoted to molecular & cellular mechanisms · 2026Article
- Adaptive evolution of Topoisomerase II triggers reproductive isolation in Drosophila.bioRxiv : the preprint server for biology · 2026Article
- MYC modulates TOP2A diffusion to promote substrate detection and activity.Nature communications · 2026Article
- Reconstitution of genome remodeling usingFrontiers in cell and developmental biology · 2026Review
- Spatial Chromatin Organization Across the Cell Cycle: Insights from Auxin-Inducible Protein Depletion.Cells · 2025Review
- Functional interplay between condensin I and topoisomerase Iiα in single-molecule DNA compaction.Nature communications · 2025Article
- Mitotic genome folding.The Journal of cell biology · 2025Review
- Inducing and Monitoring Liquid-Liquid Phase Separation by Type II Topoisomerases.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Review
- Human Topoisomerase IIα Promotes Chromatin Condensation Via a Phase Transition.bioRxiv : the preprint server for biology · 2024Article
- Mitotic chromosomes are self-entangled and disentangle through a topoisomerase-II-dependent two-stage exit from mitosis.Molecular cell · 2024Article
- Article
- Elasticity control of entangled chromosomes: Crosstalk between condensin complexes and nucleosomes.Biophysical journal · 2023Article
- Genome control by SMC complexes.Nature reviews. Molecular cell biology · 2023Review
- Methylated histones on mitotic chromosomes promote topoisomerase IIα function for high fidelity chromosome segregation.iScience · 2023Article
- Monitoring the compaction of single DNA molecules inProceedings of the National Academy of Sciences of the United States of America · 2023Article
- Super-resolution microscopy reveals the number and distribution of topoisomerase IIα and CENH3 molecules within barley metaphase chromosomes.Chromosoma · 2023Article
- DNA-Stimulated Liquid-Liquid phase separation by eukaryotic topoisomerase ii modulates catalytic function.eLife · 2022Article
- Regulation of the mitotic chromosome folding machines.The Biochemical journal · 2022Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Topoisomerase II (topo II) is one of the six proteins essential for mitotic chromatid reconstitution in vitro. It is not fully understood, however, mechanistically how this enzyme regulates this process. In an attempt to further refine the reconstitution assay, we have found that chromosomal binding of Xenopus laevis topo IIα is sensitive to buffer conditions and depends on its C-terminal domain (CTD). Enzymological assays using circular DNA substrates supports the idea that topo IIα first resolves inter-chromatid entanglements to drive individualization and then generates intra-chromatid entanglements to promote thickening. Importantly, only the latter process requires the CTD. By using frog egg extracts, we also show that the CTD contributes to proper formation of nucleosome-depleted chromatids by competing with a linker histone for non-nucleosomal DNA. Our results demonstrate that topo IIα utilizes its CTD to deliver the enzymatic core to crowded environments created during mitotic chromatid assembly, thereby fine-tuning this process.
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