ArticleTranslational vision science & technology2021
Epithelial Regeneration in Human Corneas Preserved in an Active Storage Machine.
Article in Translational vision science & technology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
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Who cites it
6 citing papers in PubMed, 11 citations in OpenAlex.
- DNA Oxidation and Expression of Repair Enzymes in Organ- Cultured Human Limbal Epithelium.International journal of molecular sciences · 2026Article
- In Vitro/Ex Vivo Release Study of a Ground Umbilical Cord Matrix Loaded with Dexamethasone.Journal of functional biomaterials · 2024Article
- Design and validation of a custom-made system to measure transepithelial electrical impedance in human corneas preserved in active storage machine.International journal of pharmaceutics: X · 2024Article
- Single cell transcriptomics reveals the heterogeneity of the human cornea to identify novel markers of the limbus and stroma.Scientific reports · 2021Article
- Key Role of Staphylococcal Fibronectin-Binding Proteins During the Initial Stage ofFrontiers in cellular and infection microbiology · 2021Article
- Ex vivo model of herpes simplex virus type I dendritic and geographic keratitis using a corneal active storage machine.PloS one · 2020Article
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Authors and funding
12 authors at 5 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Purpose: To characterize the corneal epithelium (CE) and limbal epithelium (LE) of human corneas stored in an innovative active storage machine (ASM) after a period of organ culture (OC). Methods: Corneas unsuitable for graft and stored in a standard commercial OC medium for 2 to 5 weeks were transferred into our ASM for 14 days. The ASM actively maintained an overpressure on the endothelial side (20 mm Hg) while ensuring medium renewal. We compared three modalities of storage in the ASM's epithelial chamber: (1) alternating exposure to a supplemental hormonal epithelial medium (SHEM) and air (air-lifting), (2) continuous immersion in SHEM, and (3) continuous immersion in OC medium. Passive immersion of the whole cornea in OC medium or of the CE in SHEM with or without airlifting served as controls. Paired corneas were used for better comparability. Histology, differentiation (by immunolabeling), and ultrastructure were analyzed at the end. Results: The ASM with air-lifting was most effective in regenerating a pluristratified and differentiated CE (apical ZO-1 and MUC16 staining and regeneration of the glycocalyx). In addition, the LE was stratified with preserved expression of ABCB5. The ASM with immersion in SHEM or OC medium gave a less stratified and differentiated CE. In the three control groups, the epithelia, when present, were paucistratified and less differentiated. Conclusions: In human corneas previously stored in OC, the ASM regenerates a CE with differentiation characteristics close to normal. Translational Relevance: Regeneration of the epithelium of human corneas discarded by eye banks will increase tissue availability for research.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.