ArticlePloS one2021
Prolonged ex-vivo normothermic kidney perfusion: The impact of perfusate composition.
Article in PloS one, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers, 1 of them a synthesis that pooled it.
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The trial behind it
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Who cites it
20 citing papers in PubMed, 1 synthesis or guideline pooled it, 37 citations in OpenAlex.
- Perfusate Composition and Duration ofTransplant international : official journal of the European Society for Organ Transplantation · 2022Pooled it
- Systematic production of human kidney organoids for transplantation in porcine kidneys during ex vivo machine perfusion.Nature biomedical engineering · 2026Article
- Real-time Quality Assessment of Donor Kidneys During Normothermic Machine Perfusion Using Perfusate and Urinary Biomarkers.Transplantation direct · 2026Article
- Renal Arterial Anatomy: Implications for Normothermic Machine Perfusion in Renal Transplantation.Clinical anatomy (New York, N.Y.) · 2026Article
- Vasoreactivity as a Measure of Kidney Viability During Ex Vivo Normothermic Machine Perfusion.Artificial organs · 2026Article
- An Ex Vivo Patient-Derived Tumor-Bearing Human Kidney Model Recapitulates Drug Toxicity and Metabolic Distribution.Research (Washington, D.C.) · 2026Article
- Improving outcomes in kidney transplantation through advances in donor organ perfusion.Nature reviews. Nephrology · 2025Review
- Normothermic Kidney Perfusion: Current Status and Future Perspectives.Kidney international reports · 2025Review
- Article
- Assessing Biomarkers of Porcine Kidneys under Normothermic Machine Perfusion-Can We Gain Insight into a Marginal Organ?International journal of molecular sciences · 2024Article
- Assessment of hemodynamic and blood parameters that may reflect macroscopic quality of porcine kidneys during normothermic machine perfusion using whole blood.World journal of urology · 2024Article
- Pre-transplant kidney quality evaluation using photoacoustic imaging during normothermic machine perfusion.Photoacoustics · 2024Article
- Comparison of in-gel and in-solution proteolysis in the proteome profiling of organ perfusion solutions.Clinical proteomics · 2023Article
- Current Evidence and Future Perspectives to Implement Continuous and End-Ischemic Use of Normothermic and Oxygenated Hypothermic Machine Perfusion in Clinical Practice.Journal of clinical medicine · 2023Review
- Taurine as Antioxidant in a Novel Cell- and Oxygen Carrier-Free Perfusate for Normothermic Machine Perfusion of Porcine Kidneys.Antioxidants (Basel, Switzerland) · 2023Article
- Oxygen carriers affect kidney immunogenicity duringFrontiers in transplantation · 2023Article
- 48 h Normothermic Machine Perfusion With Urine Recirculation for Discarded Human Kidney Grafts.Transplant international : official journal of the European Society for Organ Transplantation · 2023Article
- Preservation of Organs to Be Transplanted: An Essential Step in the Transplant Process.International journal of molecular sciences · 2022Review
- Review
- Renal Delivery of Pharmacologic Agents During Machine Perfusion to Prevent Ischaemia-Reperfusion Injury: From Murine Model to Clinical Trials.Frontiers in immunology · 2021Review
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Normothermic machine perfusion (NMP) of donor kidneys provides the opportunity for improved graft preservation and objective pre-transplant ex-vivo organ assessment. Currently, a multitude of perfusion solutions exist for renal NMP. This study aimed to evaluate four different perfusion solutions side-by-side and determine the influence of different perfusate compositions on measured renal perfusion parameters. Porcine kidneys and blood were obtained from a slaughterhouse. Kidneys underwent NMP at 37°C for 7 hours, with 4 different perfusion solutions (n = 5 per group). Group 1 consisted of red blood cells (RBCs) and a perfusion solution based on Williams' Medium E. Group 2 consisted of RBCs, albumin and a balanced electrolyte composition. Group 3 contained RBCs and a medium based on a British clinical NMP solution. Group 4 contained RBCs and a medium used in 24-hour perfusion experiments. NMP flow patterns for solutions 1 and 2 were similar, solutions 3 and 4 showed lower but more stable flow rates. Thiobarbituric acid reactive substances were significantly higher in solution 1 and 4 compared to the other groups. Levels of injury marker N-acetyl-β-D glucosaminidase were significantly lower in solution 2 in comparison with solution 3 and 4. This study illustrates that the perfusate composition during NMP significantly impacts the measured perfusion and injury parameters and thus affects the interpretation of potential viability markers. Further research is required to investigate the individual influences of principal perfusate components to determine the most optimal conditions during NMP and eventually develop universal organ assessment criteria.
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