Evidence map›Paper›PMID 34003582›Full record

ArticlePharmacology research & perspectives2021

Subtype selective fluorescent ligands based on ICI 118,551 to study the human β2-adrenoceptor in CRISPR/Cas9 genome-edited HEK293T cells at low expression levels.

Joëlle Goulding, Sarah J Mistry, Mark Soave, Jeanette Woolard, Stephen J Briddon, Carl W White, Barrie Kellam, Stephen J Hill

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Pharmacology research & perspectives, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.7field-weighted citation impact, top 31% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 12 citations in OpenAlex.

  1. Review
  2. Article
  3. Kinetic analysis of endogenous βBritish journal of pharmacology · 2023
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 2 institutions in 2 countries.

Joëlle GouldingDivision of Physiology, Pharmacology and Neuroscience, School of Life Sciences, University of Nottingham, Nottingham, UK.ORCID 0000-0002-6227-4483
Sarah J MistryCentre of Membrane Proteins and Receptors (COMPARE), University of Birmingham and University of Nottingham, Midlands, UK.ORCID 0000-0003-1409-0097
Mark SoaveDivision of Physiology, Pharmacology and Neuroscience, School of Life Sciences, University of Nottingham, Nottingham, UK.ORCID 0000-0002-9199-1772
Jeanette WoolardDivision of Physiology, Pharmacology and Neuroscience, School of Life Sciences, University of Nottingham, Nottingham, UK.ORCID 0000-0001-5406-6847
Stephen J BriddonDivision of Physiology, Pharmacology and Neuroscience, School of Life Sciences, University of Nottingham, Nottingham, UK.
Carl W WhiteDivision of Physiology, Pharmacology and Neuroscience, School of Life Sciences, University of Nottingham, Nottingham, UK.ORCID 0000-0002-4975-6189
Barrie KellamCentre of Membrane Proteins and Receptors (COMPARE), University of Birmingham and University of Nottingham, Midlands, UK.ORCID 0000-0003-0030-9908
Stephen J HillDivision of Physiology, Pharmacology and Neuroscience, School of Life Sciences, University of Nottingham, Nottingham, UK.ORCID 0000-0002-4424-239X
University of Nottingham · GBAustralian Research Council · AU

Funding

Medical Research Council MR/N020081/1
6 · The paper itself

Abstract

Fluorescent ligand technologies have proved to be powerful tools to improve our understanding of ligand-receptor interactions. Here we have characterized a small focused library of nine fluorescent ligands based on the highly selective β

Indexed as

Receptors, Adrenergic, beta-2Adrenergic beta-2 Receptor AntagonistsCRISPR-Cas SystemsFluorescenceGene EditingHEK293 CellsHumansLigandsPropanolaminesADRB2 protein, humanAdrenergic beta-2 Receptor AntagonistsICI 118551LigandsPropanolaminesReceptors, Adrenergic, beta-2CRISPR/Cas9 genome editingfluorescent ligandsICI 118,551ligand bindingNanoBRETβ2-adrenoceptors

Identifiers

PMID34003582
PMCPMC8130569
OpenAlexW3163248190

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.