Evidence map›Paper›PMID 34002325›Full record

ArticleVirus genes2021

MicroRNAs miR-18a and miR-452 regulate the replication of enterovirus 71 by targeting the gene encoding VP3.

Zhuo Yang, Qin Zhuo, Wen Qin, Jingbo Wang, Liyuan Wang, Po Tien

Abstract read
PubMed Publisher
In one paragraph

Article in Virus genes, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.2field-weighted citation impact, top 50% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 3 citations in OpenAlex.

  1. Article
  2. Review
  3. Role of Non-Coding RNA in Neurological Complications Associated With Enterovirus 71.Frontiers in cellular and infection microbiology · 2022
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 2 institutions in 1 country.

Zhuo YangNational Institute for Nutrition and Health, Chinese Center for Disease Control and Prevention, Beijing, 100050, China. yangzhuo@ninh.chinacdc.cn.ORCID http://orcid.org/0000-0002-6403-9356
Qin ZhuoNational Institute for Nutrition and Health, Chinese Center for Disease Control and Prevention, Beijing, 100050, China.
Wen QinNational Institute for Nutrition and Health, Chinese Center for Disease Control and Prevention, Beijing, 100050, China.
Jingbo WangNational Institute for Nutrition and Health, Chinese Center for Disease Control and Prevention, Beijing, 100050, China.
Liyuan WangNational Institute for Nutrition and Health, Chinese Center for Disease Control and Prevention, Beijing, 100050, China.
Po TienKey Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, China.
Chinese Center For Disease Control and Prevention · CNChinese Academy of Sciences · CN

Funding

Operating Project of Public Health Emergency Response Mechanism of National Institute for Nutrition and Health, CCDC 131031107000160002
6 · The paper itself

Abstract

MicroRNAs (miRNAs) are crucial in the process of host-pathogen interaction. In this study, we established a screening system for miRNAs of target genes to detect the effect of miRNAs on Enterovirus 71 (EV71) replication in rhabdomyosarcoma (RD) cells. A 3'-untranslated region (UTR) dual-luciferase assay was performed to confirm putative miRNA targets in EV71 genome. Firstly, 13 fragments of EV71 genome were inserted into the vector pMIR, and luciferase activities were analyzed to identify the putative miRNAs of target genes. The expression of the reporter protein was significantly downregulated in cells transfected with the vector containing gene VP3. Then we screened for miRNAs that might target to VP3 through online analysis software. In addition, Western blot, real-time PCR, virus titration, and morphological changes were considered to examine the effects of miRNAs on virus replication. The results suggested that miR-18a and miR-452 repress the reproduction of EV71 virus by binding to VP3. Moreover, EV71 infection also affected the expression of endogenous miR-18a and miR-452. In addition, no significant cytotoxic effects were observed. The results from this study suggest that the intracellular miRNAs may play vital roles in the host-virus interaction.

Indexed as

Enterovirus A, HumanEnterovirus InfectionsGenome, ViralHost-Pathogen InteractionsHumansMicroRNAsRNA-Binding ProteinsViral ProteinsVirus ReplicationMicroRNAsMIRN18A microRNA, humanMIRN452 microRNA, humanRNA-Binding ProteinsViral ProteinsEnterovirus 71MiRNAsRD cellVirus replicationVP3

Identifiers

PMID34002325
OpenAlexW3162137319

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.