ArticleAmino acids2021
Lyophilization and homogenization of biological samples improves reproducibility and reduces standard deviation in molecular biology techniques.
Article in Amino acids, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed.
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- Nanoparticulate protectants for the dry storage of protein therapeutics.Nanomedicine (London, England) · 2026Review
- Trientine ameliorates bleomycin-induced pulmonary fibrosis in rats through copper chelation and modulation of the CTR1/LOX/COL pathway.Inflammopharmacology · 2026Article
- Impact of the manufacturing process on the commutability of erythropoietin control materials for external quality assessment schemes.Frontiers in molecular biosciences · 2026Article
- Assessment of different preservation techniques for human skeletal muscle biopsy samples: A comparative method study on freeze-drying, RNAlater, and RNAlater-ICE.Physiological reports · 2025Article
- Cryogenic mouse tissue homogenization as an alternative to fresh-frozen biopsy use for genomics, transcriptomics, proteomics and metabolomics.Scientific reports · 2025Article
- Passive Sampling of Urban Runoff Solids to Capture Environmental DNA for the Detection of Rodents in Urban Spaces.Water environment research : a research publication of the Water Environment Federation · 2025Article
- Differential regulation during development, aging, and disease implies heart cell specific functions of the Mediator Complex.Journal of molecular and cellular cardiology plus · 2025Article
- Protocols for Extraction of miRNA from Extracellular Vesicles of Lyophilized Human Saliva Samples.International journal of molecular sciences · 2025Article
- Digestibility and palatability of extruded dog food with Aspergillus oryzae fermented soybean meal and its influence on colonic fermentation Indicators.Journal of animal science · 2025Article
- Preservation of human vascular tissue and the relevance of temperature: a narrative review.Frontiers in bioengineering and biotechnology · 2025Review
- Optimizing protein yield from growing deer antlers.Frontiers in bioengineering and biotechnology · 2025Article
- RNA Stability: A Review of the Role of Structural Features and Environmental Conditions.Molecules (Basel, Switzerland) · 2024Review
- Rapid LC-MS/MS Evaluation of Collagen and Elastin Crosslinks in Human and Mouse Lung Tissue with a Novel Bioanalytical Surrogate Matrix Approach.International journal of molecular sciences · 2024Article
- Comprehensive Overview of Bottom-Up Proteomics Using Mass Spectrometry.ACS measurement science au · 2024Review
- SARS-CoV-2 RT-LAMP in saliva: enhancing the results via a combination of cooling and specimen dilution procedure.Virusdisease · 2024Article
- Development and evaluation of a lyophilization protocol for colorimetric RT-LAMP diagnostic assay for COVID-19.Scientific reports · 2024Article
- Freeze-drying revolution: unleashing the potential of lyophilization in advancing drug delivery systems.Drug delivery and translational research · 2024Review
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Authors and funding
9 authors.
Funding
Abstract
Lyophilization is a cost-effective method for biological specimen preservation but detailed tissue-specific reference protocols are still lacking. Moreover, data are limited on the long-term stability of proteins and nucleic acids in lyophilized samples.Here, we offer lyophilization protocols for various rat and mouse tissues (kidney, heart, liver, lung, aorta, and skin) coupled with technical hints for optimal sample preparation. We demonstrate that lyophilized samples stored at 4 °C for 20 months can yield protein and RNA of similar quantity and quality to -80 °C storage, while phosphorylated proteins are preserved as well. Freeze-dried and subsequently pulverized samples can provide more consistent, more reliable data especially when investigating focal injuries, such as fibrosis. We developed a protocol for the concentration of biological solutions and achieved 20-times concentration in human peritoneal dialysis effluent solution which enables the previously unattainable detection of proteins in these samples. We established a method for water removal as well as accurate water content measurement of fecal samples, which can be valuable for gut metabolome analysis.Taken together, lyophilization is a valuable tool for the preservation of biological samples with many advantages. We aim to draw attention to the wide range of possibilities offered by freeze drying in pre-clinical or basic research.
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