ArticleBMC biology2021
Single-cell genomics unveils a canonical origin of the diverse mitochondrial genomes of euglenozoans.
Article in BMC biology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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12 citing papers in PubMed, 21 citations in OpenAlex.
- Mitochondrial Genome Reduction and Accelerated Evolution in Planktonic Foraminiferans.MicrobiologyOpen · 2026Article
- Assembly and Annotation of Kinetoplastid and Diplonemid Mitochondrial Genomes.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Euglenid Extrachromosomal DNA: Assembly and Annotation.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Comparative Analysis of Protist Communities in Oilsands Tailings Using Amplicon Sequencing and Metagenomics.Environmental microbiology · 2025Article
- Blastocrithidia nonstop mitochondrial genome and its expression are remarkably insulated from nuclear codon reassignment.Nucleic acids research · 2024Article
- Identification of diverse RNA viruses inVirus evolution · 2024Article
- The persistent homology of mitochondrial ATP synthases.iScience · 2023Article
- Recent expansion of metabolic versatility in Diplonema papillatum, the model species of a highly speciose group of marine eukaryotes.BMC biology · 2023Article
- Article
- Typical structure of rRNA coding genes in diplonemids points to two independent origins of the bizarre rDNA structures of euglenozoans.BMC ecology and evolution · 2022Article
- Mitochondrial RNA editing inComputational and structural biotechnology journal · 2022Article
- Phylogenetic Placement and Morphological Description of Three Species of Heterotrophic Euglenids (Euglenozoa) From Arizona: Scytomonas flumensis n. sp., Ploeotia protuberantia n. sp., and Hokulea pusilla comb. nov.The Journal of eukaryotic microbiologyArticle
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7 authors at 5 institutions in 5 countries.
Funding
Abstract
backgroundThe supergroup Euglenozoa unites heterotrophic flagellates from three major clades, kinetoplastids, diplonemids, and euglenids, each of which exhibits extremely divergent mitochondrial characteristics. Mitochondrial genomes (mtDNAs) of euglenids comprise multiple linear chromosomes carrying single genes, whereas mitochondrial chromosomes are circular non-catenated in diplonemids, but circular and catenated in kinetoplastids. In diplonemids and kinetoplastids, mitochondrial mRNAs require extensive and diverse editing and/or trans-splicing to produce mature transcripts. All known euglenozoan mtDNAs exhibit extremely short mitochondrial small (rns) and large (rnl) subunit rRNA genes, and absence of tRNA genes. How these features evolved from an ancestral bacteria-like circular mitochondrial genome remains unanswered.
resultsWe sequenced and assembled 20 euglenozoan single-cell amplified genomes (SAGs). In our phylogenetic and phylogenomic analyses, three SAGs were placed within kinetoplastids, 14 within diplonemids, one (EU2) within euglenids, and two SAGs with nearly identical small subunit rRNA gene (18S) sequences (EU17/18) branched as either a basal lineage of euglenids, or as a sister to all euglenozoans. Near-complete mitochondrial genomes were identified in EU2 and EU17/18. Surprisingly, both EU2 and EU17/18 mitochondrial contigs contained multiple genes and one tRNA gene. Furthermore, EU17/18 mtDNA possessed several features unique among euglenozoans including full-length rns and rnl genes, six mitoribosomal genes, and nad11, all likely on a single chromosome.
conclusionsOur data strongly suggest that EU17/18 is an early-branching euglenozoan with numerous ancestral mitochondrial features. Collectively these data contribute to untangling the early evolution of euglenozoan mitochondria.
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