ArticleMicrobial cell factories2021
Biosynthesis of pinene in purple non-sulfur photosynthetic bacteria.
Article in Microbial cell factories, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 32 citations in OpenAlex.
- Metabolic engineering of endogenous MEP pathway for enhanced lycopene production inSynthetic and systems biotechnology · 2026Article
- Research progress on biosynthesis and regulation of monoterpenoid compounds.Natural products and bioprospecting · 2026Review
- A non-classical biosynthetic route enables tocopherol synthesis in bacteria.Nature metabolism · 2026Article
- Metabolic engineering for improved heterologous pinene production in the chloroplast of Phaeodactylum tricornutum.Microbial cell factories · 2026Article
- Distribution of Genetic Determinants Associated with CRISPR-Cas Systems and Resistance to Antibiotics in the Genomes of Archaea and Bacteria.Microorganisms · 2025Article
- Rational engineering ofFrontiers in microbiology · 2024Article
- Application of valencene and prospects for its production in engineered microorganisms.Frontiers in microbiology · 2024Review
- Multidimensional optimization for accelerating light-powered biocatalysis in Rhodopseudomonas palustris.Biotechnology for biofuels and bioproducts · 2023Article
- Alternate gene expression profiling of monoterpenes in Hymenocrater longiflorus as a novel pharmaceutical plant under water deficit.Scientific reports · 2022Article
- Toward improved terpenoids biosynthesis: strategies to enhance the capabilities of cell factories.Bioresources and bioprocessing · 2022Review
- Screening and engineering of high-activity promoter elements through transcriptomics and red fluorescent protein visualization inSynthetic and systems biotechnology · 2021Article
- Metabolic engineering of Deinococcus radiodurans for pinene production from glycerol.Microbial cell factories · 2021Article
- Photosynthetic Conversion of COFrontiers in bioengineering and biotechnology · 2021Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
Abstract
backgroundPinene is a monoterpene, that is used in the manufacture of fragrances, insecticide, fine chemicals, and renewable fuels. Production of pinene by metabolic-engineered microorganisms is a sustainable method. Purple non-sulfur photosynthetic bacteria belong to photosynthetic chassis that are widely used to synthesize natural chemicals. To date, researches on the synthesis of pinene by purple non-sulfur photosynthetic bacteria has not been reported, leaving the potential of purple non-sulfur photosynthetic bacteria synthesizing pinene unexplored.
resultsRhodobacter sphaeroides strain was applied as a model and engineered to express the fusion protein of heterologous geranyl diphosphate synthase (GPPS) and pinene synthase (PS), hence achieving pinene production. The reaction condition of pinene production was optimized and 97.51 μg/L of pinene was yielded. Then, genes of 1-deoxy-D-xylulose 5-phosphate synthase, 1-deoxy-D-xylulose 5-phosphate reductoisomerase and isopentenyl diphosphate isomerase were overexpressed, and the ribosome binding site of GPPS-PS mRNA was optimized, improving pinene titer to 539.84 μg/L.
conclusionsIn this paper, through heterologous expression of GPPS-PS, pinene was successfully produced in R. sphaeroides, and pinene production was greatly improved by optimizing the expression of key enzymes. This is the first report on pinene produce by purple non-sulfur photosynthetic bacteria, which expands the availability of photosynthetic chassis for pinene production.
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