ArticleBMC biotechnology2021
Comparative evaluation of different molecular methods for DNA extraction from individual Teladorsagia circumcincta nematodes.
Article in BMC biotechnology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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5 citing papers in PubMed, 12 citations in OpenAlex.
- Ambient temperature storage of individual parasitic nematode larvae for whole genome sequencing.PLoS neglected tropical diseases · 2026Article
- Comparative performance of traditional and commercial DNA extraction methods for fish gut microbiota analysis.Molecular biology reports · 2026Article
- Multi-omics reveals effects of diet FNDF/starch level on growth performance and rumen development of Hu sheep.Frontiers in microbiology · 2025Article
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- Discovery of Early-BranchingFrontiers in microbiology · 2022Article
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe purpose of this study was to develop a reliable DNA extraction protocol to use on individual Teladorsagia circumcincta nematode specimens to produce high quality DNA for genome sequencing and phylogenetic analysis. Pooled samples have been critical in providing the groundwork for T. circumcincta genome construction, but there is currently no standard method for extracting high-quality DNA from individual nematodes. 11 extraction kits were compared based on DNA quality, yield, and processing time.
results11 extraction protocols were compared, and the concentration and purity of the extracted DNA was quantified. Median DNA concentration among all methods measured on NanoDrop 2000™ ranged between 0.45-11.5 ng/μL, and on Qubit™ ranged between undetectable - 0.962 ng/μL. Median A260/280 ranged between 0.505-3.925, and median A260/230 ranged - 0.005 - 1.545. Larval exsheathment to remove the nematode cuticle negatively impacted DNA concentration and purity.
conclusionsA Schistosoma sp. DNA extraction method was determined as most suitable for individual T. circumcincta nematode specimens due to its resulting DNA concentration, purity, and relatively fast processing time.
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