ArticleMicrobial biotechnology2021
Role of phenazine-enzyme physiology for current generation in a bioelectrochemical system.
Article in Microbial biotechnology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 32 citations in OpenAlex.
- Boosting phenazine-1-carboxylic acid (PCA) yield in Pseudomonas aeruginosa and assessing antifungal performance of PCA-loaded mesoporous carriers.Archives of microbiology · 2026Article
- WoundMx: multiplexed detection of wound infection biomarkers with a multimodal sensor system based on laser-induced graphene.NPJ 2D materials and applications · 2026Article
- Genomic and Functional Characterization ofFrontiers in microbiology · 2026Article
- Microbial phenazines: biosynthesis, structural diversity, evolution, regulation, and biological significance.Microbiology and molecular biology reviews : MMBR · 2025Review
- The TonB-Dependent Transport System Facilitates the Uptake of Inorganic Metal Mediators in Pseudomonas putida KT2440 in a Bioelectrochemical System.Microbial biotechnology · 2025Article
- Dynamic synthesis and transport of phenazine-1-carboxylic acid to boost extracellular electron transfer rate.Nature communications · 2025Article
- Engineering of bespoke photosensitiser-microbe interfaces for enhanced semi-artificial photosynthesis.Chemical science · 2024Review
- Electricity generation byEngineering microbiology · 2024Article
- A phenazine-inspired framework for identifying biological functions of microbial redox-active metabolites.Current opinion in chemical biology · 2023Review
- Screening of natural phenazine producers for electroactivity in bioelectrochemical systems.Microbial biotechnology · 2023Article
- The Influence of Hydrodynamic Conditions in a Laboratory-Scale Bioreactor onMicroorganisms · 2022Article
- Evaluation of extracellular electron transfer in Pseudomonas aeruginosa by co-expression of intermediate genes in NAD synthetase production pathway.World journal of microbiology & biotechnology · 2022Article
- Microbial Electrochemical Technologies for Sustainable Nitrogen Removal in Marine and Coastal Environments.International journal of environmental research and public health · 2022Review
- Using structure-function relationships to understand the mechanism of phenazine-mediated extracellular electron transfer iniScience · 2021Article
- Role of phenazine-enzyme physiology for current generation in a bioelectrochemical system.Microbial biotechnology · 2021Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
Pseudomonas aeruginosa produces phenazine-1-carboxylic acid (PCA) and pyocyanin (PYO), which aid its anaerobic survival by mediating electron transfer to distant oxygen. These natural secondary metabolites are being explored in biotechnology to mediate electron transfer to the anode of bioelectrochemical systems. A major challenge is that only a small fraction of electrons from microbial substrate conversion is recovered. It remained unclear whether phenazines can re-enter the cell and thus, if the electrons accessed by the phenazines arise mainly from cytoplasmic or periplasmic pathways. Here, we prove that the periplasmic glucose dehydrogenase (Gcd) of P. aeruginosa and P. putida is involved in the reduction of natural phenazines. PYO displayed a 60-fold faster enzymatic reduction than PCA; PCA was, however, more stable for long-term electron shuttling to the anode. Evaluation of a Gcd knockout and overexpression strain showed that up to 9% of the anodic current can be designated to this enzymatic reaction. We further assessed phenazine uptake with the aid of two molecular biosensors, which experimentally confirm the phenazines' ability to re-enter the cytoplasm. These findings significantly advance the understanding of the (electro) physiology of phenazines for future tailoring of phenazine electron discharge in biotechnological applications.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.