Evidence map›Paper›PMID 33999923›Full record

ArticlePloS one2021

Generation of the short TRIM32 isoform is regulated by Lys 247 acetylation and a PEST sequence.

Juncal Garcia-Garcia, Katrine Stange Overå, Waqas Khan, Eva Sjøttem

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in PloS one, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.2field-weighted citation impact, top 52% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 2 citations in OpenAlex.

  1. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 1 institution in 1 country.

Juncal Garcia-GarciaDepartment of Medical Biology, Autophagy Research Group, University of Tromsø-The Arctic University of Norway, Tromsø, Norway.
Katrine Stange OveråDepartment of Medical Biology, Autophagy Research Group, University of Tromsø-The Arctic University of Norway, Tromsø, Norway.
Waqas KhanDepartment of Medical Biology, Autophagy Research Group, University of Tromsø-The Arctic University of Norway, Tromsø, Norway.
Eva SjøttemDepartment of Medical Biology, Autophagy Research Group, University of Tromsø-The Arctic University of Norway, Tromsø, Norway.ORCID 0000-0003-2668-1708
UiT The Arctic University of Norway · NO

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

TRIM32 is an E3 ligase implicated in diverse biological pathways and pathologies such as muscular dystrophy and cancer. TRIM32 are expressed both as full-length proteins, and as a truncated protein. The mechanisms for regulating these isoforms are poorly understood. Here we identify a PEST sequence in TRIM32 located in the unstructured region between the RING-BBox-CoiledCoil domains and the NHL repeats. The PEST sequence directs cleavage of TRIM32, generating a truncated protein similarly to the short isoform. We map three lysine residues that regulate PEST mediated cleavage and auto-ubiquitylation activity of TRIM32. Mimicking acetylation of lysine K247 completely inhibits TRIM32 cleavage, while the lysines K50 and K401 are implicated in auto-ubiquitylation activity. We show that the short isoform of TRIM32 is catalytic inactive, suggesting a dominant negative role. These findings uncover that TRIM32 is regulated by post-translational modifications of three lysine residues, and a conserved PEST sequence.

Indexed as

AcetylationCell LineHEK293 CellsHumansLysineMuscular Dystrophies, Limb-GirdleProtein BindingProtein IsoformsProtein Processing, Post-TranslationalTranscription FactorsTripartite Motif ProteinsUbiquitinationUbiquitin-Protein LigasesLysineProtein IsoformsTranscription FactorsTRIM32 protein, humanTripartite Motif ProteinsUbiquitin-Protein Ligases

Identifiers

PMID33999923
PMCPMC8128265
OpenAlexW3163254069

What OpenQuestion holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.