Evidence map›Paper›PMID 33997821›Full record

ArticleSTAR protocols2021

Comprehensive analysis of liver macrophage composition by flow cytometry and immunofluorescence in murine NASH.

Sabine Daemen, Mandy M Chan, Joel D Schilling

Open access · goldAbstract read
In one paragraph

Article in STAR protocols, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.

0numbers the graph read from it
0cells of the map it votes in
36citing papers in PubMed
5.6field-weighted citation impact, top 3% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

36 citing papers in PubMed, 55 citations in OpenAlex.

  1. Article
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  6. [Characterization of liver macrophage subsets in different mouse models of metabolic associated steatohepatitis].Zhonghua gan zang bing za zhi = Zhonghua ganzangbing zazhi = Chinese journal of hepatology · 2026
    Article
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  10. The role of VISTA engagement in limiting neutrophil-mediated inflammation.Journal of immunology (Baltimore, Md. : 1950) · 2025
    Article
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  16. Article
  17. Review
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  19. Molecular imaging of macrophage composition and dynamics in MASLD.JHEP reports : innovation in hepatology · 2024
    Article
  20. Effect of theHeliyon · 2024
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 1 institution in 1 country.

Sabine DaemenDiabetes Research Center, Washington University School of Medicine, St. Louis, MO 63110, USA.
Mandy M ChanDepartment of Medicine, Washington University School of Medicine, St. Louis, MO 63110, USA.
Joel D SchillingDiabetes Research Center, Washington University School of Medicine, St. Louis, MO 63110, USA.
Washington University in St. Louis · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Recently, it has become evident that macrophage diversity increases in the liver during the pathogenesis of non-alcoholic steatohepatitis (NASH). Here, we provide a detailed protocol for the analysis of liver macrophage subsets in mice with non-alcoholic fatty liver disease (NAFLD) and early NASH using flow cytometry and immunofluorescence (IF). These methods can be used to assess the composition and localization of macrophage subsets during NASH. For complete details on the use and execution of this protocol, please refer to Daemen et al. (2021).

Indexed as

Flow CytometryLiverMacrophagesNon-alcoholic Fatty Liver DiseaseAnimalsDisease Models, AnimalMiceFlow Cytometry/Mass CytometryImmunologyMetabolismMicroscopyModel OrganismsSingle Cell

Identifiers

PMID33997821
PMCPMC8102804
OpenAlexW3158121248

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.