Evidence map›Paper›PMID 33997817›Full record

ArticleSTAR protocols2021

Mapping of domain-mediated protein-protein interaction by SPOT peptide assay.

Xiaoyi Mao, Godwin Sokpor, Jochen Staiger, Huu Phuc Nguyen, Tran Tuoc

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in STAR protocols, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.2field-weighted citation impact, top 48% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 4 citations in OpenAlex.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 1 institution in 1 country.

Xiaoyi MaoUniversity Medical Center Goettingen, 37077 Goettingen, Germany.
Godwin SokporRuhr University of Bochum, 44791 Bochum, Germany.
Jochen StaigerUniversity Medical Center Goettingen, 37077 Goettingen, Germany.
Huu Phuc NguyenRuhr University of Bochum, 44791 Bochum, Germany.
Tran TuocRuhr University of Bochum, 44791 Bochum, Germany.
Ruhr University Bochum · DE

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Identification of peptides mediating protein-protein interaction (PPI) is crucial for understanding the function of interlinked proteins in cellular processes and amino acid-associated diseases. Traditional PPI assays are laborious, involving the generation of many truncated proteins. SPOT peptide assay allows high-throughput detection of domains essential for PPI by synthesizing several hundred peptides on a cellulose membrane. Here, we present a rapid SPOT peptide protocol for identifying the binding motifs, which mediate interaction between the chromatin remodeling factors BAF155/BAF170 and the epigenetic factor Kdm6b. For complete details on the use and execution of this protocol, please refer to Narayanan et al. (2015).

Indexed as

Peptide LibraryProtein Interaction MappingProteomicsDNA-Binding ProteinsHeLa CellsHumansJumonji Domain-Containing Histone DemethylasesProtein Interaction Domains and MotifsTranscription FactorsDNA-Binding ProteinsJumonji Domain-Containing Histone DemethylasesKDM6B protein, humanPeptide LibrarySMARCC1 protein, humanSMARCC2 protein, humanTranscription FactorsHigh Throughput ScreeningProtein BiochemistryProtein expression and purificationProteomics

Identifiers

PMID33997817
PMCPMC8100624
OpenAlexW3159102987

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.